雌激素替代治疗对去卵巢雌性大鼠萘性白内障晶状体上皮细胞凋亡相关蛋白Bcl-2和Bax表达的影响  

Effects of estrogen replacement therapy on expression of apoptosis related protein Bcl-2 and Bax in lens epithelial cells of naphthalene induced cataract in ovariectomized female rats

作　　者：康刚劲[1] 李平华[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,400016

出　　处：《重庆医学》2010年第19期2561-2564,共4页Chongqing medicine

基　　金：泸州医学院科研基金资助项目(04113)

摘　　要：目的观察雌二醇及雌二醇联合孕酮对去卵巢雌性大鼠萘性白内障晶状体上皮细胞凋亡相关蛋白Bcl-2和Bax表达的影响。方法将32只健康成年雌性SD大鼠随机分为4组,即对照组、卵巢切除组、雌二醇组(卵巢切除加雌二醇替代治疗组)、雌二醇加孕酮组(卵巢切除加雌二醇和孕酮替代治疗组)。术后2周各组均用萘混悬液灌胃并行裂隙灯显微镜检查,观察各组大鼠晶状体变化;灌胃6周后处死大鼠,采用放射免疫法检测血清中雌二醇和孕酮浓度,免疫组化(S-P)法观察晶状体上皮细胞(LECs)中凋亡相关蛋白Bcl-2和Bax的表达。结果雌二醇组、雌二醇加孕酮组以及对照组晶状体浑浊程度较卵巢切除组轻,出现时间晚;雌二醇组和雌二醇加孕酮组血清雌二醇与孕酮水平接近对照组(P>0.05);除卵巢切除组外,3组间Bcl-2蛋白和Bax蛋白表达差异无统计学意义(P>0.05);卵巢切除组血清雌二醇与孕酮水平显著低于其余3组(P<0.01),Bcl-2蛋白表达阳性率较其余3组低,Bax蛋白表达阳性率则较其余3组高(P<0.05)。结论抑制晶状体上皮细胞凋亡可能是雌二醇对萘处理去卵巢雌性大鼠晶状体保护作用的机制之一;雌二醇可能通过下调Bax蛋白表达和上调Bcl-2蛋白表达抑制LECs的凋亡而阻止晶状体混浊。Objective To investigate the effect of estradiol and estradiol combined progesterone on expression of apoptosis related protein Bcl-2 and Bax in lens epithelial cells of naphthalene induced cataract in ovariectomized female rats.Methods Thirty-two adult femal Sprague-Dawley rats were divided into four groups randomly：Control,ovariectomy,estradiol（ovariectomy and estradiol replacement therapy）,estradiol and progesterone（ovariectomy combined estradiol and progesterone replacement therapy）.After 2 weeks of operation,each group received naphthalene for 6 weeks through a stomach tube.The changes of lenses were observed with slit-lamp microscope.After 6 weeks of naphthalene administration,all rats were killed and serum estradiol and progesterone levels were determined with radioimmunoassay in each rat.The rats lenses were taken out to observe the expression of apoptosis related protein Bcl-2 and Bax were observed with SP imunohistochemical technique.Results In control,estradiol,as well as estradiol and progesterone groups,opacity of lens were similar,and more slight and late than ovariectomy group.In addition,there was no significiant difference in the expression of Bcl-2 and Bax protein among estradiol,estradiol and progesterone,also control group.On the contrary,in ovariectomy group,the expression of Bcl-2 protein decreaced,Bax protein increaced than other groups（P0.05）.The level of serum estradiol and progesterone in ovariectomy group decreased significiantly as compared with control group（P0.01）;but estradiol,estradiol and progesterone groups expressed similarly to control group（P0.05）,and there was no significiant difference among estradiol,estradiol and progesterone groups（P0.05）.Conclusion In naphthalene induced cataract in ovariectomized female rats,anti-apoptosis may be one of mechanism of estradiol protection on lens.Estradiol maybe can up-regulate expression of Bcl-2 protein and down-regulate expression of Bax protein to inhibit apoptosis of LECs,and prevent opacity of lens.Estradio

关 键 词：雌激素替代治疗 雌二醇 晶状体上皮细胞 凋亡 BCL-2/BAX 

分 类 号：R776.1[医药卫生—眼科]