迷走神经刺激对大鼠海马神经元和星形胶质细胞的影响  

Responses of neurons and astrocytes in rat hippocampus following vagus nerve stimulation

作　　者：古慕峰[1] 韩群颖[1] 张永杰[1] 朱敏[1] 李雷[1] 唐敏峰[1] 

机构地区：[1]南京医科大学,江苏南京210029

出　　处：《江苏预防医学》2010年第5期16-19,共4页Jiangsu Journal of Preventive Medicine

基　　金：江苏省分子神经生物重点实验室开放科研基金资助项目(KJS03055)

摘　　要：目的:研究迷走神经刺激(VNS)对海马星形胶质细胞和神经元的影响,阐明海马在迷走神经抑制癫痫中的作用。方法:利用免疫组织化学法,观察VNS前后,海马胶原纤维酸性蛋白(GFAP)和Fos蛋白的表达变化。结果:VNS治疗后,海马Fos、GFAP和Fos/GFAP双标细胞数,明显低于癫痫组。结论:VNS可能通过抑制海马星形胶质细胞Fos蛋白表达发挥抑制癫痫作用。Objective：To investigate the quantitative and morphological changes of astroeytes and neurons in hippoeampus after vagus nerve stimulation（VNS）. Methods： Immunohistochemical technique was used to observe Fos and glial fibrillary acidic portein （GFAP） immunoreactive cells and the double-labled cells in hippocampus after VNS. Results： Fos and GFAP immunoreaetive cells and Fos/GFAP double-labled ceils were significantly decreased in hippocampus after VNS. Conclusion： The results from above suggested that the decreased expression of c-Fos gene in astrocyte after VNS may play a role in antiepilepsy effect.

关 键 词：迷走神经刺激 海马 星形胶质细胞 癫痫 

分 类 号：R-33[医药卫生]