芳香酰胺类化合物的选择性水解菌初筛模型的研究  

A screening system for enantioselective hydrolysis of arylamides

作　　者：胡蓉[1] 章银军[1] 应向贤[1] 汪钊[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《生物加工过程》2010年第5期64-68,共5页Chinese Journal of Bioprocess Engineering

摘　　要：建立了一种快速筛选选择性水解手性芳香酰胺类化合物微生物的方法。在中性或弱碱性条件下,KMnO4能氧化芳香胺,但对芳香乙酰胺没有氧化效果。KMnO4被芳香胺还原而褪色,通过褪色程度来快速测定菌的酶活力及手性选择性。以1-苯基乙酰胺为例,得出最佳筛选体系：最大吸收波长为525 nm;1-苯基乙胺在0.04~5 mmol/L的浓度范围内与其ΔA525符合朗伯比尔定律;反应温度为60℃,反应时间为20 m in。通过该模型筛到5株选择性的菌株,R-选择性的菌株4株,S-选择性的菌株1株。A novel enantioselective amidase screening system was developed.Under neutral or alkaline condition,KMnO4 could oxidize arylamines but could not oxidize arylacetamide.The enzyme activity and the enantioselective of the microorganism were detected by the fading of KMnO4.The enantioselectivities of amidase were evaluated by 1-phenyl-acetamide.The conditions of optimal screening system was as follows： the maximum absorptive at 525 nm,the concentration range from 0.04 mmol/L to 5 mmol/L,temperature at 60 ℃,and reaction time 20 min.By using the method,5 strains with enantioselective amidase were obtained,in which 4 showed（R）-specific avtivity and 1 showed（S）-aspecificity.

关 键 词：芳香酰胺类化合物 筛选 对映选择性 

分 类 号：Q814[生物学—生物工程]