基于纳米ZnO-壳聚糖-酪氨酸酶生物传感器对邻苯二酚的测定  

Biosensor based on nano-ZnO-chitosan-tyrosinase for the determination of catechol

作　　者：韩瑞霞[1] 邱岩岩[1] 艾仕云[1] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《化学传感器》2010年第3期54-58,共5页Chemical Sensors

基　　金：国家自然科学基金(No.20775044);山东省自然科学基金(No.Y2006B20)

摘　　要：以Zn(Ac)2和N,N-二甲基甲酰胺为原料,采用水热合成法在160℃下合成出了纳米ZnO,并用X-射线衍射、透射电镜表征了所制备纳米ZnO的结构,同时以制备的纳米ZnO固定酪氨酸酶制备了多酚生物传感器,结果表明高等电点的纳米ZnO在很大程度上促进了酪氨酸酶与电极之间的直接电子传递,能够有效地用于多酚的测定。Using Zn（Ac）2 and N,N-Dimethylformamide as raw materials, nanometersized ZnO was prepared at 160 ℃ by hydrothermal reaction method. The X-ray diffraction （XRD） and Transmission electron microscopy （TEM） were used to analyse the structure of nanometersized ZnO. At the same time, the application of nanometersized ZnO using in modifying electrodes to detect the polyphenol was preliminarily studied, the results showed that the high-isoelectric point nano-ZnO promoted the direct electron transfer between the tyrosinase and the electrode to a large extent, could be effectively used for the determination of polyphenol.

关 键 词：纳米ZNO 水热法 多酚 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]