偏侧咀嚼对大鼠咬肌组织Ca^(2+)质量分数、Ca^(2+)-ATP酶活性影响的研究  

A study on the influence of unilateral chewing on Ca^(2+) concentration and Ca^(2+)-ATP enzyme activity in rat masseter muscle

作　　者：刘钊[1,2] 徐龙博[3] 丁婷婷[1,2] 祁冬[1,2] 林雪芬[1,2] 汲平[1,2] 

机构地区：[1]山东大学口腔医院修复科 [2]山东大学口腔生物医学实验室,济南250012 [3]济宁市口腔医院儿童牙病科,山东济宁272045

出　　处：《中国实用口腔科杂志》2010年第9期535-537,共3页Chinese Journal of Practical Stomatology

基　　金：山东省自然科学基金资助项目(Y2006C126)

摘　　要：目的研究偏侧咀嚼后大鼠咬肌Ca2+质量分数、Ca2+-ATP酶活性以及咬肌超微结构的变化。方法 2009年9—10月于山东大学动物实验中心选取8周龄雌性Wistar大鼠10只,随机分为对照组和偏侧咀嚼组,每组各5只。偏侧咀嚼组大鼠拔除左侧上颌磨牙;对照组大鼠模拟拔牙操作,不拔除牙齿。4周后处死各组大鼠,切取浅层咬肌组织。采用火焰原子吸收分光光度法测定咬肌组织中Ca2+质量分数;Ca2+-ATP酶活性运用超微量Ca2+-ATP酶试剂盒测定;透射电镜观察咬肌组织超微结构的变化。结果偏侧咀嚼组大鼠拔牙侧Ca2+质量分数较非拔牙侧及对照组均有明显升高(P<0.05);偏侧咀嚼组大鼠拔牙侧Ca2+-ATP酶活性较非拔牙侧及对照组均有明显降低(P<0.01)。透射电镜下观察发现,偏侧咀嚼组大鼠咬肌组织的超微结构与对照组相差较大,损伤表现拔牙侧重于非拔牙侧。结论偏侧咀嚼后大鼠拔牙侧咬肌Ca2+质量分数升高、Ca2+-ATP酶活性降低以及咬肌超微结构出现病理性变化。Objective To study the influence of unilateral chewing on Ca^2＋ concentration and Ca^2＋-ATP enzyme activity and ultrastructure in rat masseter muscle. Methods The 10 rats were randomly divided into（A）normal control group and（B）unilateral chewing experimental group. The Ca^2＋ concentration was determined by atomic absorption spectrophotometry;the activity of the Ca^2＋-ATP enzyme was determined by super-micro volume Ca^2＋-ATP enzyme Kit. The ultrastructure of the rat masseter muscle was observed under TEM. Results The Ca^2＋ concentration of the extraction side of group B had significant difference compared with the non-extraction side（P = 0.02 0.05）,group A（P 〈0.05）;Ca^2＋-ATP enzyme activity of group B extraction side were higher than its non-extraction side （P 〈0.01）in group A（P 〈0.01）. The ultrastructural changes of the rat masseter muscle under TEM were：the extraction side of group B had more severe pathological manifestations than its non-extraction side. Conclusion Higher Ca^2＋ concentration,lower Ca^2＋-ATP enzyme activity and pathological changes of ultrastructure appear in rat masseter muscle after unilateral chew.

关 键 词：偏侧咀嚼 咬肌 CA2+ CA2+-ATP酶 

分 类 号：R78[医药卫生—口腔医学]