染料木素酯化修饰物与ctDNA的相互作用  

Study on the Interactions of Genistein Esterified Derivatives with ctDNA

作　　者：刘蓉[1] 邓泽元[1] 余燕影[2] 李先和[2] 李强[2] 曹树稳[1,2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌市南京东路235号330047 [2]南昌大学化学系

出　　处：《光谱实验室》2010年第5期1698-1704,共7页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(No:20562008);长江学者和创新团队发展计划(IRT0540)资助项目

摘　　要：在pH7.2的Tris-HCl缓冲溶液中,采用紫外光谱和荧光光谱法研究了2种新型染料木素酯化修饰物,染料木素7-乙酰阿魏酸酯(GenA)和染料木素7,4′-二-乙酰阿魏酸酯(GenDA)与小牛胸腺DNA(ctDNA)的相互作用。随着ctDNA的加入,GenA与GenDA的紫外吸收和荧光光谱的强度均发生不同程度的降低。ctDNA对化合物的荧光猝灭为静态猝灭过程。在293K时化合物与ctDNA的结合常数分别为2.81×106L.mol-1和1.19×104L.mol-1。同时通过I-离子效应、离子强度、DNA熔点、粘度法等研究证实,在该实验条件下,GenA与ctDNA之间具有较强的作用,主要以嵌插方式结合;GenDA主要以沟槽方式与ctDNA作用。The interactions of two novel genistein esterified derivatives,genistein 7-acetylferulic acid ester（GenA）and genistein 7,4＇-di-acetylferulic acid ester（GenDA）,with calf thymus DNA（ctDNA）in Tris-HCl buffer（pH 7.2）were studied using UV-Vis spectroscopy and fluorescene spectroscopy.The decrease in the intensity of UV-Vis absorption and fluorescence spectra of GenA and GenDA were observed upon addition of ctDNA.The fluorescence of GenA and GenDA quenched by ctDNA may be mainly governed by static quenching mechanisms.The binding constants（KA）of GenA and GenDA with DNA at 293K were 2.81×10^6L·mol^-1 and 1.19×10^4L·mol^-1,respectively.Besides,studies on effects of I-,effects of ionic strength,DNA melting temperature and viscosity mesurements showed that there was a relatively strong interaction between GenA and ctDNA.The binding modes of GenA and GenDA with ctDNA were evaluated to be intercalative and groove binding,respectively.

关 键 词：染料木素 酯化修饰物 小牛胸腺DNA 作用方式 

分 类 号：O657.32[理学—分析化学]