辅酶依赖型和不依赖型甘油脱水酶的比较  

Comparison between the B_(12)-Dependent and Independent Glycerol Dehydratase

作　　者：杨仲丽[1] 方柏山[1] 余劲聪[1] 王庆花[1] 李梓君[1] 

机构地区：[1]华侨大学工业生物技术福建省高等学校重点实验室,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2010年第5期542-547,共6页Journal of Huaqiao University(Natural Science)

基　　金：国家高技术研究发展(863)计划项目(2006AA020103);国家自然科学基金资助项目(20676048;30770059)

摘　　要：实验比较源自肺炎克氏杆菌(K.pneumoniae)和丁酸梭状芽孢杆菌(C.butyricum)中的甘油脱水酶(GDHt)的辅酶依赖特性.采用生物信息学的方法,对两种酶的异同点进行分析,发现两种酶结构上相似性较低、亲缘性较远、分属于两个不同的家族.SMART数据库搜索结果表明,C.butyricum中不依赖辅酶的甘油脱水酶及其再激活酶,分别属于gly-Radical和Radical-SAM家族,多序列比对得到的一段富含半胱氨酸残基C**C**C的保守序列正是其共同特征.In this paper,the coenzyme B12 dependence of glycerol dehydratase between Klebsiella pneumoniae and Clostridia butyricum was compared.Their differences and similarities were analyzed with bioinformatics.Results showed that these two enzymes belonged to two different families with low similarities.Searching the SMART database and the result also shows that the B12-independent glycerol dehydratase from Clostridia butyricum and its reactivate enzyme belong to gyl-Radical family and Radical-SAM family respectively,and the conserved sequence which is rich in half-cysteine obtained by multiple alignments is their common feature.

关 键 词：肺炎克氏杆菌 丁酸梭状芽孢杆菌 辅酶 甘油脱水酶 维生素B12 依赖性 

分 类 号：Q55[生物学—生物化学]