类人猪H3N2流感病毒与禽H5流感病毒重组:一个潜在的公共威胁  

Reassortant between human-like H3N2 and avian H5 subtype influenza A viruses in pigs:apublic health risk

作　　者：王广美[1] 丛彦龙[1] 丁壮[1] 常爽[1] 张泉鹏[1] 孙玉章[1] 王晓莉[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2010年第9期1213-1216,1222,共5页Chinese Journal of Veterinary Science

基　　金：吉林大学农学部引进人才科研启动基金(4305050102B6)

摘　　要：将2007年7月—2008年10月从吉林省猪群中分离到的3株H3N2亚型流感病毒,分别命名为A/swine/Ji-lin/A/2007(Sw/Jilin/A/07),A/swine/Jilin/B/2007(Sw/Jilin/B/07)和A/swine/Jilin/C/2008(Sw/Jilin/C/08)。结合已知猪流感病毒(SIV)内部基因序列,设计合成6对特异性引物,通过RT-PCR技术分别扩增出6段内部基因,并与GenBank中相关序列进行比较,分析3株流感病毒内部基因的遗传进化关系。结果表明:在PB2、PB1、PA和NP的进化树上,3株H3N2分离株处于近代人源谱系内;在M和NS进化树中,Sw/Jilin/A/07和Sw/Jilin/B/07处于近代人源谱系内,而Sw/Jilin/C/08与禽源谱系内的H5亚型病毒亲缘关系最近。Three H3N2 influenza viruses were isolated from pig farms in Jilin province,China from July 2007to October 2008,named A/swine/Jilin/A/2007（Sw/Jilin/A/07）,A/swine/Jilin/B/2007（Sw/Jilin/B/07）,A/swine/Jilin/ C/2008（Sw/Jilin/C/08）,respectively.The six internal segments of the three influenza viruses were amplified by specific primers which were designed according to the corresponding gene sequences in GenBank,and then sequenced.The phylogenetic relationships among swine H3N2 viruses prevalent in Jilin province compared to selected reference strains available in the GenBank were estimated from the nucleotide sequences of each internal viral gene. The results showed that the PB2,PB1,PA,and NP genes of the three virus isolates in this study belonged to the contemporary human lineage.In the M and NS trees,Sw/Jilin/A/07 and Sw/Jilin/B/07still clustered in contemporary human lineage,whereas Sw/Jilin/C/08was most related to the H5subtype viruses in the avian lineage

关 键 词：猪流感病毒 H3N2 遗传分析 

分 类 号：S852.65[农业科学—基础兽医学]