高效液相色谱法同时测定饲料中的呋喃唑酮、呋喃西林、呋喃妥因、呋喃它酮药物残留量  

DETERMINATION OF THE PESTICIDE RESIDUES OF FURAZOLIDONUM,FURACILIN,TITROFURANTOINE AND FURALTADON IN FEED BY HPLC

作　　者：曹鹏[1,2] 耿金培 尹大路 阮新 李佩暖 梁君妮 

机构地区：[1]烟台出入境检验检疫局,山东烟台264001 [2]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2010年第3期424-427,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：山东省自然科学基金资助项目(ZR2009DM032)

摘　　要：本研究建立了用固相萃取-高效液相色谱法同时测定饲料中呋喃唑酮、呋喃西林、呋喃妥因和呋喃它酮药物残留量的方法。样品中的残留药物用乙腈提取,经正己烷和酸性氧化铝净化,高效液相色谱法检测。呋喃西林、呋喃妥因、呋喃它酮的检出限为0.25 mg/kg,定量限为0.5 mg/kg;呋喃唑酮检出限为0.12 mg/kg,定量限为0.25 mg/kg,四种药物的平均添加回收率均大于70%,相对标准偏差<10%。本方法简便、准确、快速,适用于同时测定饲料中的四种硝基呋喃类药物。A method has been developed to determine the pesticide residues of furazolidonum,furacilin,titrofurantoine and furaltadon in feed by HPLC at the same time.The pesticide residues are extracted by acetonitrile,purified by hexane and alumine,and detected by HPLC.The LOD and LOQ for furacilin,titrofurantoine and furaltadon are 0.25 mg/kg and 0.5 mg/kg.The LOD and LOQ for furazolidonum are 0.12 mg/kg and 0.25 mg/kg.The average recoveries of the four pesticides are more than 70%,and the RSD are less than 10%.The method is simple,accurate and rapid,it can be used for determining the pesticide residues of four nitrofurans in feed at the same time.

关 键 词：高效液相色谱法 饲料 呋喃唑酮 呋喃西林 呋喃妥因 呋喃它酮 

分 类 号：S859[农业科学—临床兽医学]