大鼠颌下腺放射性损伤的细胞学模型的建立  

Research of the model of salivary gland radiation injury of rat

作　　者：高洋[1,2] 柯学平[1,2] 杨建荣 

机构地区：[1]南京医科大学口腔医学研究所 [2]南京医科大学附属口腔医院口腔颌面外科,南京210029

出　　处：《口腔生物医学》2010年第3期140-142,共3页Oral Biomedicine

基　　金：江苏省自然科学基金(BK2007182)

摘　　要：目的建立能用于细胞增殖诱导研究的涎腺放射性损伤细胞学模型。方法对离体培养的大鼠颌下腺细胞分别采用不同剂量的^(60)Coγ射线照射,倒置显微镜下观察细胞生长形态,MTS法检测其细胞的增殖率。结果与未照射组相比,3Gy以下剂量,细胞增殖率未见明显改变,5Gy以上的剂量,细胞仍有部分增殖,但增殖率明显下降(P<0.01),而7Gy以上的剂量照射则表现为细胞的大范围死亡。结论一次性大剂量照射对离体培养下的大鼠颌下腺细胞的形态和增殖率均造成一定的影响,5Gy剂量照射下的鼠颌下腺细胞可用于细胞增殖诱导研究的放射性损伤细胞学模型。Objective To explore the modeling of cellular radiation injury of rat submandibular gland.Methods With different doses of ^（60）Co γ-ray irradiation for rat submandibular gland cells,the morphology of the submandibular gland cell and the cell proliferation after radiation were observed through microscope.Results With dose below 3 Gy,cell proliferation rates were not found significant change.With dose of 5 Gy and above,significant changes in cell proliferation rates were spotted（P0.01）,while more than 7 Gy dose can lead to extensive cell death.Conclusions With irradiation for one time,the morphology and proliferation rates of rat submandibular gland cells had been affected,and the cell proliferation under 5 Gy dose continued to be stable.

关 键 词：颌下腺 放射性损伤 细胞模型 

分 类 号：R730.55[医药卫生—肿瘤]