海南长臂猿线粒体D-loop区序列分析及种群复壮  

Mitochondrial DNA D-loop sequence analysis and population rejuvenation of Hainan gibbons (Nomascus hainanus)

作　　者：李志刚[1] 魏辅文[2] 周江[1,2] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001 [2]中国科学院动物研究所,北京100101

出　　处：《生物多样性》2010年第5期523-527,共5页Biodiversity Science

基　　金：香港嘉道理农场暨植物园奖学金项目

摘　　要：海南长臂猿(Nomascus hainanus)是世界上最濒危的灵长类动物之一,但目前有关海南长臂猿的种群遗传学方面的信息以及种群复壮所面临的困难未见报道。为更好地保护该极危物种,作者以粪便为研究材料,首次在分子生物学水平上测定了海南长臂猿1个群体(B群)共6个个体的线粒体D-loop区基因序列。结果显示:202bp的D-loop区基因共检测到5个变异位点,4个单倍型,单倍型多样性(h)为0.6000,核苷酸多样性(π)为0.00829,表明海南长臂猿B群的遗传多样性较低;与此同时,海南长臂猿还面临着种群数量过小,性比失衡,以及栖息地质量低下等严峻的问题。The Hainan gibbon（Nomascus hainanus） is one among the most endangered primates globally according to Critically Endangered List of IUCN.However,there has been no report on its genetic information and the difficulties of its population restoration.This study represents the first time survey of genetic diversity using mitochondrial D-loop sequences from faecal sampling of six individuals within one group.Five variable sites were detected in the 202 bp D-loop sequences and 4 haplotypes were defined.The haplotype diversity（h） and nucleotide diversity（π） were 0.6000 and 0.00829,respectively,meaning the genetic diversity was low.Furthermore,this critically endangered species also face other threatening factors,such as small population size,biased sex ratio and low habitat quality.

关 键 词：Nomascus hainanus 粪便DNA 线粒体D-LOOP 遗传多样性 

分 类 号：Q953[生物学—动物学]