Assessing the estrogenic potency in a Portuguese wastewater treatment plant using an integrated approach  

作　　者：Mário S.Diniz Rita Maurício Mira Petrovic Maria J.López De Alda Leonor Amaral Isabel Peres Damiá Barceló Fernando Santana 

机构地区：[1]Universidade Nova de Lisboa,Faculdade de Ciencias e Tecnologia,REQUIMTE,Dep.de Quimica [2]Universidade Nova de Lisboa,Faculdade de Ciencias e Tecnologia,Dep.de Ciencias e Engenharia do Ambiente [3]Department of Environmental Chemistry-IIQAB-CSIC c/Jordi Girona

出　　处：《Journal of Environmental Sciences》2010年第10期1613-1622,共10页环境科学学报（英文版）

基　　金：supported by the Portuguese Foundation for Science and Technology(FCT/MC) to its financial support to the project"Comprehensive Assessment of Impacts of Endocrine Disruptors Compounds from Urban Wastewater"(No.POCT/36303/MGS/2000);the authors Ph.D grant(No.SFRH/BD/3098/2000,SFRH/BD/3093/2000)

摘　　要：The estrogenic potency of a wastewater treatment plant （WWTP） was evaluated using chemical and biological analyses, which showed that after the station treatment processes some of the selected endocrine disruptor compounds （EDCs） were still present in the treated effluent （e.g., bisphenol A, alkylphenols, estrone）. Thus, the most common endocrine EDCs were identified and quantified and the overall estrogenicity of the treated effluent assessed by integrating the results. Male goldfish （Carassius auratus） were used as biological indicators in a 28-day experiment. Vitellogenin （Vtg）, gonadosomatic and hepatosomatic indices, steroids （17β-estradiol and 11-ketotestosterone） and histopathology were biomarkers used in fish to evaluate WWTP treated effluent estrogenicity, in combination with instrumental analyses. The results showed a significant increase （P 〈 0.01） in plasma and liver Vtg, which were significantly correlated （r = 0.66; P 〈 0.01）. The gonadosmatic index was significantly （P 〈 0.01） reduced in exposed fish. The steroid analyses revealed significant elevations in 17β-estradiol and depressed 11-ketotestosterone concentrations. The histological examinations show changes in exposed fish gonads, such as regressed testes and in some cases （43% to 75%） the development of ovo-testis in fish exposed to 50% and 100% treated effluent.The estrogenic potency of a wastewater treatment plant （WWTP） was evaluated using chemical and biological analyses, which showed that after the station treatment processes some of the selected endocrine disruptor compounds （EDCs） were still present in the treated effluent （e.g., bisphenol A, alkylphenols, estrone）. Thus, the most common endocrine EDCs were identified and quantified and the overall estrogenicity of the treated effluent assessed by integrating the results. Male goldfish （Carassius auratus） were used as biological indicators in a 28-day experiment. Vitellogenin （Vtg）, gonadosomatic and hepatosomatic indices, steroids （17β-estradiol and 11-ketotestosterone） and histopathology were biomarkers used in fish to evaluate WWTP treated effluent estrogenicity, in combination with instrumental analyses. The results showed a significant increase （P 〈 0.01） in plasma and liver Vtg, which were significantly correlated （r = 0.66; P 〈 0.01）. The gonadosmatic index was significantly （P 〈 0.01） reduced in exposed fish. The steroid analyses revealed significant elevations in 17β-estradiol and depressed 11-ketotestosterone concentrations. The histological examinations show changes in exposed fish gonads, such as regressed testes and in some cases （43% to 75%） the development of ovo-testis in fish exposed to 50% and 100% treated effluent.

关 键 词：Carassius auratus endocrine disruptors VITELLOGENIN STEROIDS INTERSEX 

分 类 号：X703[环境科学与工程—环境工程]