^(12)C^(6+)离子束辐照紫苏干种子当代效应  

Effects of Irradiation with ^(12)C^(6+) Ions on M1 Characters of Perilla frutescens(L.) Britt

作　　者：武振华[1,2,3] 张红[1,2,3] 王新宇[4] 薛林贵[5] 牛炳韬[4] 赵旭[5] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]中国科学院重离子束辐射生物医学重点实验室,甘肃兰州730000 [3]甘肃省重离子束辐射医学应用基础重点实验室,甘肃兰州730000 [4]兰州大学生命科学学院细胞生物学研究所,甘肃兰州730000 [5]兰州交通大学化学与生物工程学院,甘肃兰州730070

出　　处：《原子核物理评论》2010年第3期335-340,共6页Nuclear Physics Review

基　　金：甘肃省自然科学基金资助项目(0803RJZA074)~~

摘　　要：利用兰州重离子研究装置(HIRFL)提供的12C6+离子束辐照紫苏干种子(辐照剂量为40,80和120Gy,剂量率4Gy/min),探讨了重离子束辐照对紫苏M1代的生物学效应。结果发现,经不同剂量的12C6+离子束辐照后,紫苏种子的发芽率、发芽势、存活率、株高、分枝数、单株产量和千粒重等生物学性状均发生了变化,其中发芽势、单株产量和千粒重随辐照剂量的提高而降低,且有明显的剂量效应关系,但发芽率、大田成活率、株高和分枝数却随辐照剂量的增大,呈现出明显的"抛物线"趋势;紫苏幼苗根尖细胞的微核率和染色体畸变率随辐照剂量增加呈线性增加关系。这表明:12C6+重离子束辐照紫苏种子,具有明显的当代损伤效应,在本试验剂量范围内,低剂量辐照对发芽率和成活率有促进作用。To investigate the M1 biological effects of heavy ions on Perilla frutescens（L.）,its dry seeds of it were irradiated by 12C6＋ with the dose of 0,40,80 or 120 Gy respectively at the rate of 4 Gy/min delivered by the Heavy Ion Research Facility in Lanzhou（HIRFL）.The results showed that biological characters such as germinating rate,germinating potentiality,survival rate,plant height,numbers of branch,yield per plant and 1000-grain weight were changed after irradiation.Moreover,the germinating potentia-lity,yield per plant and 1000-grain weight decreased in a dose-dependent manner.The germinating rate,survival rate,plant height and numbers of branch exhibited obviously ＂parabola＂ trends with irradiation doses.The frequency of micronucleus and chromosomal aberration in root-tip cells of Perilla frutescens（L.）was observed ＂linear rise＂ trend with the increase of radiation dosage.Data suggest that exposure with low-dose 12C6＋ to seeds of Perilla frutescens（L.）has obvious injury effects at the first generation,and the low-dose irradiation has apparent promotive function to the germinating rates and survival rates in the range of irradiation dosage at this experiment.

关 键 词：紫苏 12C6+离子束 诱变育种 生物性状 染色体畸变 

分 类 号：Q691.5[生物学—生物物理学] Q691.8