固定化酶催化合成半乳糖寡糖  

Synthesis of Galato oligosaccharide by Immobilized β Galactosidase

作　　者：王筱兰[1] 魏东芝[1] 崔玉敏[1] 俞俊棠[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室

出　　处：《华东理工大学学报（自然科学版）》1999年第2期144-146,159,共4页Journal of East China University of Science and Technology

摘　　要：以壳聚糖固定化米曲霉β－半乳糖苷酶，连续催化合成半乳糖寡糖（ＧＯＳ）。在填充床固定化酶反应器中，研究底物浓度、ｐＨ、反应温度和停留时间对ＧＯＳ连续合成的影响。最佳反应条件为：底物浓度４００ｇ／Ｌ，反应温度５０°Ｃ，ｐＨ５．８，停留时间３５ｍｉｎ，产物的最高得率为６２％。β galactosidase from Aspergillus oryzae was immobilized, using chitosan as the carrier, The galacto oligosaccharide was synthesized continuously in packed bed reactor. The effect of substrate concentration, pH value, reaction temperature and retention time on production of GOS were studied. The optimal reaction condition were determined and the product yield was 62%.

关 键 词：半乳糖寡糖 催化 固定化酶 填充床反应器 合成 

分 类 号：Q533.03[生物学—生物化学]