左旋千金藤啶碱对缺氧纹状体和海马脑片Ca^(2+)/CaMPKⅡ和PKA活性的影响  

EFFECT OF STEPHOLIDINE ON Ca 2+ /CaM PKⅡAND PKA ACTIVITIES IN STRIATAL AND HIPPOCAMPAL SLICES

作　　者：孙亚锋[1] 唐放鸣[1] 王然[1] 张光毅[1] 金国章[1] 

机构地区：[1]中科院上海药物所

出　　处：《徐州医学院学报》1999年第2期89-92,共4页Acta Academiae Medicinae Xuzhou

基　　金：江苏省自然科学基金;江苏省教委自然科学基金

摘　　要：目的研究左旋千金藤啶碱（ＳＰＤ）对Ｃａ２＋／ＣａＭＰＫⅡ活性的影响。方法采用大鼠海马及纹状体脑片体外缺血模型，用放射性３２Ｐ－ＡＴＰ掺入法测定Ｃａ２＋／ＣａＭＰＫＩ活性。结果体外培养的纹状体和海马脑片在缺血３０ｍｉｎ后，Ｃａ２＋／ＣａＭＰＫⅡ活性均明显下降，纹状体ＰＫＡ活性增加，于缺血前１０ｍｉｎ加入不同浓度ＳＰＤ后，二酶活性较单纯缺血均有明显改变。Objective To study the effect of stepholidine (SPD) on Ca 2+ /CaM PKⅡand PKA activities.Methods On the in vitro models of rat striatal and hippocampal slices, the Ca 2+ /CaM PKⅡand PKA activities were determined by 32 P isotope incorporation.Results After ischemia for 30min, the Ca 2+ /CaM PKⅡ activity of rat striatal and hippocampal slices decreased significantly, and the PKA activity of rat striatal slice increased. Following the use of SPD 10 min before ischemia, the enzyme activities were significantly ameliorated as compaired with that in simple ischemia.Conclusion SPD has protective effect on the activities of PKA in rat striatal slice and of Ca 2+ /CaM PKⅡin rat striatal and hippocampal slices.

关 键 词：脑缺血 多巴胺受体 左旋千金藤啶碱 钙 PKA 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R364.12[医药卫生—临床医学]