睑板腺癌P53基因和人乳头状瘤病毒HPV-16的检测  

DETECTION OF P53 GENE AND HUMAN PAPILLOMAVIRUS HPV16 IN MEIBOMIAN GLAND CARCINOMAS

作　　者：严瑛[1] 牛膺筠[1] 纪建中[1] 罗兵[1] 

机构地区：[1]青岛医学院

出　　处：《青岛医学院学报》1999年第1期7-9,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科委资助

摘　　要：目的探讨睑板腺癌发生的分子生物学机制。②方法采用多聚酶链反应－限制性片段长度多态性方法，对３４例眼睑组织（睑板腺癌和霰粒肿各１７例）中Ｐ５３基因２４８位点进行检测，并用ＰＣＲ技术检测其中人乳头状瘤病毒（ＨＰＶ）致癌型ＨＰＶ－１６ＤＮＡ片段。③结果２９．５％的睑板腺癌中有Ｐ５３基因的突变，霰粒肿中则未检测到，两者比较差异有显著性（Ｐ＝０．０２２）。Ｐ５３基因突变与睑板腺癌临床病理特征无关（Ｐ＞０．０５）。睑板腺癌及霰粒肿中均未检测到ＨＰＶ－１６ＤＮＡ片段。④结论Ｐ５３基因突变可能在睑板腺癌发生的早期起一定作用，而与临床病理特征无关。Objective To inquire into the molecular biological mechanisms of the occurrence of meibomian gland carcinomas. Methods Using polymerase chain reactionrestriction fragment length polymorphisms(PCRRFLP) method, we studied 248 points in 34 samples ( including 17 meibomian gland carcinomas,17 chalazions). DNA segment of HPV16 was also detected by PCR technique. Results 29.5% meibomian gland carcinoma were shown to contain point mutation in P53 gene, no point mutation was found in chalazions, the difference was significant (P=0.022).No significant correlation was found between the P53 gene mutations and the clinical pathological manifestations of meibomian gland carcinomas (P>0.05). No HPV16 DNA was found in meibomian gland carcinomas. Conclusion P53 gene mutations might play roles in the early stage of the development of meibomian gland carcinomas. No correlation between P53 gene mutations and clinical manifestations was found.

关 键 词：睑板腺癌 乳头状瘤病毒 P53基因 HPV-16 

分 类 号：R739.710.2[医药卫生—肿瘤]