甘糖酯对脑缺血-再灌注损伤大鼠海马谷氨酸转运体功能的影响  

EFFECT OF PROPLYLENE GLUCOL MANNURATE SULPHATE ON GLUTAMATE TRANSPORTER FUNCTION OF HIPPOCAMPUS IN ISCHEMIAREPERFUSION INJURED RATS

作　　者：任晓燕[1,2,3] 徐珞[1,2,3] 张玉敏[1,2,3] 葛宝林 曲忠森[1,2,3] 刘洪明 

机构地区：[1]青岛医学院病理生理学教研室 [2]青岛医学院生理学教研室 [3]泰山医学院附属医院微循环研究所

出　　处：《青岛医学院学报》1999年第1期32-33,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金

摘　　要：目的探讨甘糖酯对脑缺血－再灌注损伤大鼠海马谷氨酸转运体功能的影响。②方法采用大鼠三血管夹闭、松夹制作脑缺血－再灌注损伤模型，利用海马突触膜颗粒对３Ｈ－Ｌ－谷氨酸摄入量的测定，观察了甘糖酯对谷氨酸转运体功能的影响，同时测定了脑组织丙二醛（ＭＤＡ）含量及超氧化物歧化酶（ＳＯＤ）活性的变化。③结果缺血－再灌注损伤时，大鼠海马谷氨酸转运体功能及ＳＯＤ活性明显降低，ＭＤＡ明显升高，与对照组比较差异均有显著性（ｔ＝３．６９１～５．４０６，Ｐ均＜０．０１）；应用甘糖酯组大鼠海马谷氨酸转运体功能及ＳＯＤ活性显著提高，ＭＤＡ含量降低，与缺血－再灌注损伤组相比，差异均有显著性（ｔ＝２．９３８～３．１２７，Ｐ均＜０．０５）。④结论甘糖酯可能通过清除自由基的作用，改善大鼠脑组织缺血－再灌注损伤时海马谷氨酸转运体的功能。Objective To investigate the effect of proplylene glucol mannurate sulphate( PGMS) on glutamate transporter function of hippocampus in ischemiareperfusion injured (I120R30) rats. Methods The brain ischemiareperfusion model of rat was made by clipping and releasing three arteries.Glutamate transporter function was studied by assay of 3HLglutamate uptake in hippocampus synaptosomes. MDA contents and SOD activities were detected by spectrophotometry. Results The glutamate transporter function and SOD activities of hippocampus in ischemiareperfusion rats were significantly decreased(t=4.482,3.691,P<0.01), contents of MDA were increased(t=3.067,P<0.05) compared with control group. Glutamate transporter function and SOD activity in PGMS group were increased(t=3.127,2.938,P<0.05), contents of MDA were decreased(t=3.067,P<0.05) compared with ischemiareperfusion rats. Conclusion PGMS can improve glutamate transporter function of ischemiareperfusion rats through the elimination of free radicals.

关 键 词：甘糖酯 谷氨酸转运体 再灌注损伤 大鼠 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R364.12[医药卫生—临床医学]