大豆异黄酮对去势大鼠学习记忆能力改善作用  

Soy germ phytoestrogens improve spatial memory performance in ovariectomized rats

作　　者：潘妹霞[1] 李卓能 肖本熙[1] Victor Yeung Ruo-Jun Xu 

机构地区：[1]广东省人民医院(广东省医学科学院)营养科,广州510080 [2]武汉市疾病控制中心 [3]香港大学生命科学院

出　　处：《中国公共卫生》2010年第10期1265-1267,共3页Chinese Journal of Public Health

摘　　要：目的探讨雌激素和大豆异黄酮对去势大鼠空间学习记忆能力的影响及其可能作用机制。方法将28只去势SD大鼠随机分为4组,分别给予基础膳食,基础膳食中添加低、高剂量异黄酮(0.4,1.6 g/kg)和雌激素(0.15 g/kg);12周后,以水迷宫法检测大鼠空间学习记忆能力,并检测海马区脑源性神经营养因子(Brain-derived neurotrophic factor,BD-NF)和酪氨酸激酶B(tyrosine kinase receptors B,TrkB)的表达。结果低、高剂量异黄酮组和雌激素组大鼠的平均逃避潜伏期分别为(21.20±4.41),(19.61±4.90),(10.51±1.92)s,游泳总路程分别为(3.62±0.69),(3.76±0.60),(2.43±0.57)m,明显低于对照组的(37.77±8.25)s和(5.87±1.05)m;雌激素和高剂量大豆异黄酮明显提高海马区BDNF水平及其受体TrkB的mRNA表达;未发现异黄酮对大鼠子宫和阴道内膜的雌激素样作用。结论大豆异黄酮可改善去势大鼠的空间学习记忆能力,其作用可能与大鼠海马区BDNF及其受体表达有关。Objective To determine the effects of estradiol and soy germ phytoestrogens on spatial learning and memory performance in ovariectomized rats and to explore the possible mechanisms.Methods Ovariectomized Sprague-Dawley rats were randomly divided into four groups and assigned one of the following diets：basic diet,basic diet supplemented with phytoestrogens（0.4,1.6 g/kg diet） or 17β-estradiol（0.15 g/kg diet） for 12 weeks.The rats were evaluated for their spatial learning and memory performance with Morris water maze task.The brain-derived neurotrophic factor（BDNF） and its tyrosine kinase receptors B（TrkB）receptor expression were measured.Results The rats supplemented with phytoestrogens and estradiol performed significantly better in spatial memory acquisition and retention compared to the controls.Escape latency（s） in rats supplemented with low-phytoestrogens（21.20±4.41）,high-phytoestrogens（19.61±4.90） or estradiol（10.51±1.92） was significantly lower than that of the control（37.77±8.25）.Trip distance（m） in rats supplemented with low-phytoestrogens（3.62±0.69）,high-phytoestrogens（3.76±0.60） or estradiol（2.43±0.57） was significantly shorter than that of the control（5.87±1.05）.Estradiol or the high dose of phytoestrogens treatment significantly increased the BDNF expression and mRNA level for TrkB receptors.Phytoestrogens did not show any significant estrogenic-effect on the vaginal and uteri.Conclusion Phytoestrogens improve spatial memory performance in ovariectomized rats.The memory enhancement effect may relate to the increased expression of BDNF and its TrkB receptor in the hippocampus.

关 键 词：大豆异黄酮 雌激素 学习记忆 脑源性神经营养因子(BDNF) 

分 类 号：R151[医药卫生—营养与食品卫生学]