饮水型砷暴露人群淋巴细胞DNA损伤影响因素分析  

Assessment of DNA damage and its influential factor in peripheral blood lymphocytes of individuals exposed to drinking water arsenic

作　　者：田凤 韩光[1] 梁江[1] 高怡[1] 裴秋玲[1] 

机构地区：[1]山西医科大学公共卫生学院毒理学教研室,山西太原030001

出　　处：《中国公共卫生》2010年第10期1271-1272,共2页Chinese Journal of Public Health

基　　金：山西省回国人员基金(200527);山西省自然科学基金(20051094);山西省科技攻关项目(2006031087-11)

摘　　要：目的探讨饮水型砷暴露地区人体外周血淋巴细胞DNA的损伤。方法应用彗星试验,检测54例地砷病区有皮损患者(病例组)和51名无皮损者(对照组)外周血淋巴细胞DNA损伤水平。采集研究对象的尿液检测尿砷水平。调查研究对象年龄、性别、吸烟、饮酒、农药接触史等。结果病例组和对照组彗星尾长分别为(12.34±5.22),(7.35±3.67)μm,差异有统计学意义(P<0.05);2组尿砷分别为(1.61±0.41),(1.52±0.55)ng/1 000 g肌酐,差异无统计学意义(P>0.05);Logistic回归分析结果显示,吸烟和饮水年限为DNA损伤的危险因素。未发现饮酒对淋巴细胞DNA的损伤有影响。结论吸烟和饮水年限是DNA损伤的危险因素,彗星试验可作为砷污染地区人群健康监测的早期指标。Objective To evaluate the DNA damage in peripheral blood lymphocytes of individuals exposed to drinking water arsenic.Methods Comet assay was used to evaluate the DNA damage in peripheral blood lymphocytes of 54 individuals with skin lesion and 51 without skin lesion.Urinary arsenic of all subjects were measured.The data on age,sex,smoking,alcohol drinking and history of pesticide exposure of all subjects were collected with a questionnaire.Results The results of the comet assay showed that the tail lengths of groups with and without skin lesions were 12.34±5.22 μm and 7.35±3.67 μm with a significant difference（P0.05）.Urinary arsenic contents were 1.61±0.41 and 1.52±0.55 ng/1 000 g creatinine without significant difference（P0.05）.Smoking and duration of using well were the risk factors of DNA damage.While the effects of alcohol consumption on DNA damage was not observed.Conclusion Our study suggests that smoking and duration of using well are the risk factors of DNA damage.Comet assay can be used as a possible earlier index of the genetic damage of cell in arsenic exposed area.

关 键 词：砷 DNA损伤 彗星试验 

分 类 号：R394.2[医药卫生—医学遗传学]