基于mtDNA-COⅡ基因序列对中国南海裸胸鳝属鱼类分子系统进化关系的研究  

Molecular Phylogenetic Relationships among Six Species in Moray Eels Gymnothorax sp in South China Sea by Cytochrome Oxidase Ⅱ Gene Sequences

作　　者：齐兴柱[1] 尹绍武[1] 张本[1] 霍蕊[1] 杜民[1] 

机构地区：[1]海南大学热带生物资源教育部重点实验室,海南海口570228

出　　处：《水产科学》2010年第10期605-609,共5页Fisheries Science

基　　金："十一五"国家科技支撑计划重点项目(2007BAD29B03);国家自然科学基金资助项目(40966003);海南省教育厅高等学校科研项目(Hjkj2010-13);海南省自然科学基金资助项目(808122);海南热带水生生物技术重点实验室开放基金资助项目(shkyjj0801)

摘　　要：采用聚合酶链式反应直接测序法,获得细斑裸胸鳝、黄纹裸胸鳝、云纹裸胸鳝、黑点裸胸鳝、褐裸胸膳5种裸胸鳝属鱼类的细胞色素氧化酶Ⅱ（cytochrome oxidaseⅡ,COⅡ）基因序列（691 bp）。结合GenBank上蠕纹裸胸鳝COⅡ同源序列,采用多个生物软件对6种裸胸鳝属鱼类序列变异和碱基组成进行分析,共发现196个核苷酸位点存在变异（28.36%）;计算了它们的相对遗传距离、转换/颠换比等遗传信息指数,6种裸胸鳝属鱼类的序列差异为0.012~0.199,其中云纹裸胸鳝与褐裸胸鳝的序列差异最大,为0.199,细斑裸胸鳝与黄纹裸胸鳝序列的差异最小,为0.012;总体来说,序列中的转换明显多于颠换,转换和颠换的比值（Ts/Tv）为2.7;以长鳝为外群构建NJ、ME、MP系统树,长鳝分为独立的一支,6种裸胸鳝为另外一支,分为3个平行进化的分支。The cytochrome oxidaseⅡ genes of Gymnothorax fimbriatus,G.pseudothyrsoideus,G.chilospilus,G.melanospilus,and G.hepaticus in the South China sea were amplified by the polymerase chain reaction and direct sequence analysis to clarify the phylogenetic relationships in the genus Gymnothorax（moray eels）.The DNA sequences were all found to be 691bp,and the genetic information indexes including Kimura-2 parameter genetic distance and Ts/Tv ratios were generated among 5 species and G.kidako obtained from Genebank by a suite of biology softwares.Of the 691 aligned base pairs,196 pairs were parsimony informative,comprising 28.36 % of the total base pairs.The maximum pairwise nucleotide divergence value among all the taxa was 0.119 between G.chilospilus and G.hepaticus and the minimum value 0.012 between G.fimbriatus and G.pseudothyrsoideus.The average pairwise nucleotide divergenc evalue among all taxa was 0.169.Transitions were found to be more common than transversions.The average transition/transversion ratio among all pairwise comparisons was 2.7,suggesting that the sequences have not reached saturation.With S.sathete as the designated outgroups,phylogenetic trees were constructed based on the neighbor-joining（NJ）,Minimum Evolution（ME） and Maximum-parsimony（MP） methods.Finally,the NJ tree,the Me tree and the MP tree Clustering map shows：the six species of Gymnothorax on the test are in the same branch.There are three parallel evolutionary sister clades existing（with high bootstrap value support） in the six species.However,S.sathete is in the other branch.

关 键 词：裸胸鳝属 COⅡ基因 系统进化 

分 类 号：Q959[生物学—动物学]