利用栽培稻基因组DNA对非洲野生稻进行染色体荧光原位杂交分析  

Fluorescence in situ Hybridization of Oryza schweinfurthian Chromosomes with Genomic DNA from O.sativa

作　　者：邱小芬[1] 刘虹[1,2] 覃瑞[1,2] 陈雁[1,2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]南方少数民族地区生物资源保护与综合利用工程中心,武汉430074

出　　处：《华中农业大学学报》2010年第5期537-540,共4页Journal of Huazhong Agricultural University

基　　金：国家民委科研基金(05ZN011);中南民族大学自然科学基金项目(YZY09002;YZZ10002)资助

摘　　要：以地高辛标记的栽培稻基因组(基因组为AA)DNA为探针,对非洲野生稻(基因组为BBCC)的体细胞染色体进行荧光原位杂交分析,研究AA染色体组和BBCC染色体组之间的关系,同时对杂交后的染色体进行同源染色体配对。结果表明:栽培稻A基因组和非洲野生稻基因组有较高的同源性,其中高度重复DNA序列在栽培稻和非洲野生稻间具有保守性。Genomic in situ hybridization（GISH）analysis on Oryza schweinfurthian with digoxigenin-labelled genomic DNA from O.sativa was used to study the relationship between the A genome of O.sativa and BC genome of O.schweinfurthian.Karyotype analysis was made based on the similar band patterns of the hybridization signal.The result showed that A genome of cultivated rice had higher homology with genome of African wild rice,including repetitive DNA sequence conserved between cultivated rice and African wild rice.

关 键 词：栽培稻 非洲野生稻 基因组原位杂交(GISH) 

分 类 号：S511.035.3[农业科学—作物学]