新铁炮百合品种沈香一号的幼胚培养研究  

Study on Immature Embryo Culture of Lilium formolongi cv.Shenxiang No.1

作　　者：陈丽静[1] 马爽[1] 张晓光[1] 张丽[1] 钟鸣[1] 明军[2] 李天来[1] 陶承光[3] 

机构地区：[1]沈阳农业大学辽宁省生物技术重点实验室/设施园艺省部共建教育部重点实验室,沈阳110866 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]辽宁省农业科学院,沈阳110161

出　　处：《湖北农业科学》2010年第10期2348-2352,共5页Hubei Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)项目(2006AA100109);中国博士后科学基金第四十七批面上资助项目(20100471471);辽宁省教育厅项目(L2010495)

摘　　要：以新铁炮百合沈香一号(Lilium formolongi cv.Shenxiang No.1)的幼胚为试材,筛选适宜百合幼胚萌发的诱导培养基,结果表明,适宜的诱导培养基为1/2MS,萌发时间在接种后的22～43d之间。6-BA和NAA的浓度比与新铁炮百合幼胚的发芽率之间呈现出显著的相关性,在一定范围内,6-BA和NAA的浓度比越大,胚的萌发率越低。试验筛选出适宜新铁炮百合胚分化不定芽的诱导培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,继代增殖培养基为MS+6-BA1.0mg/L+NAA0.1mg/L及MS+6-BA2.0mg/L+NAA0.4mg/L。新铁炮百合试管苗易出现玻璃化现象,玻璃化程度与培养基的水势、环境湿度和外源激素的浓度有关。The immature embryos of Lilium formolongi cv.Shenxiang No.1 were cultured in the study.Results indicated that the best sprouting medium of the immature embryo was 1/2MS and the germination time was between 22 to 43 days after inoculation.A negative correlation was found between the germination percentage and the concentration ratio of 6-BA and NAA within limits.MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L was good for adventitious buds,while MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L and MS＋6-BA 2.0 mg/L＋NAA 0.4 mg/L was beneficial to propagation efficiency in a long run.There was a high degree of vitrifaction in the lily plantlets after subculture,which was influenced by water potential,environmental humidity and the concentration of exogenous hormone.

关 键 词：新铁炮百合 幼胚培养 玻璃化 

分 类 号：Q949.71[生物学—植物学] Q944.46