慢性肝病患者血中白蛋白基因表达研究  

The research of chronic hepatic disease patient's blood albumin gene expression

作　　者：许鸣[1] 张智[1] 张珍[1] 李泽泳[1] 

机构地区：[1]广东省第二人民医院消化内科,广东广州510317

出　　处：《中国医学工程》2010年第3期1-3,9,共4页China Medical Engineering

基　　金：广东省自然科学基金项目(编号:05003906)

摘　　要：目的研究慢性肝病患者外周血白蛋白mRNA表达与病变进程的关系。方法采用荧光定量PCR检测慢性肝病患者血清HBVDNA;采用逆转录巢式荧光定量PCR检测慢性肝病患者外周血单个核细胞(PBMNC)中白蛋白mRNA基因表达。结果 46例慢性乙型肝炎患者中,血清HBVDNA阳性26例,阳性率为56.5%。HBVDNA浓度为(3.6±3.4)×108copies/mL;而外周血单个核细胞白蛋白mRNA阳性17例,阳性率为40.0%。37例肝炎肝硬化患者中,血清HBVDNA阳性28例,阳性率为75.7%。HBVDNA浓度为(4.7±2.8)×108copies/mL;外周血单个核细胞白蛋白mRNA阳性12例,阳性率为32.4%。40例健康人外周血单个核细胞中的白蛋白mRNA阳性2例,阳性率为5%。慢性肝炎肝硬化患者血清HBVDNA明显高于慢性乙型肝炎患者(Р<0.05);慢性乙肝活动期和肝硬化失代偿期外周血单个核细胞中的白蛋白mRNA阳性率显著升高。结论血清HBVDNA与慢性肝病患者病变活动和进展密切相关;外周血单个核细胞中的白蛋白mRNA表达与肝脏损害相关,二者可作为慢性肝病病情进展临床疗效评价的重要指标。【Objective】To research the connection of mRNA expression in peripheral blood albumin and disease processes in chronic hepatic disease.【Methods】We detected the serum HBV DNA through RT-PCR method.Taking nested reverse transcriptase PCR method to detect peripheral blood mononuclear cells（PBMNC） gene expression of albumin mRNA in chronic hepatic disease patients.【Results】In the 46 cases of chronic hepatitis b patients,there were 26 cases of positive of HBV-DNA（positive rate 56.5%）.HBV-DNA concentration were（3.6 ± 3.4） × 108copies/mL.And there were 17 cases of peripheral blood mononuclear cells albumin positive（positive rate 40.0%）.In 37 cases of hepatitis and hepatocirrhosis,there were 28 cases of HBV-DNA positive（positive rate 75.7%）.HBV-DNA concentration were（4.7 ± 2.8） × 108copies/mL.There were 12 cases of peripheral blood mononuclear cells positive for albumin mRNA（positive rate 32.4%）.In 40 cases of healthy volunteers the peripheral blood mononuclear cells of albumin mRNA infection were 2 cases（positive rate 5%）.The chronic hepatitis and hepatocirrhosis patient＇s HBVDNA were significantly higher than chronic hepatitis B（Р 0.05）.Chronic hepatitis B in the active stage and hepatocirrhosis dysfunction stage the peripheral blood mononuclear cells of albumin mRNA was significantly higher.【Conclusion】HBVDNA in serum was closely related to the activity and progress in chronic hepatic disease.The albumin mRNA expression of peripheral blood mononuclear cell was related to liver damage.Both can be used as the important index of clinical evaluation in the progression of chronic liver disease.

关 键 词：慢性乙型肝炎 HBVDNA 白蛋白MRNA 肝硬化 

分 类 号：R575[医药卫生—消化系统]