凡纳滨对虾血细胞体外免疫活性保存技术的研究  

ESTABLISHMENT OF HAEMOCYTE IMMUNE ACTIVITY KEEPING METHODS IN VITRO OF LITOPENAEUS VANNAMEI

作　　者：谢鹏[1] 潘鲁青[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《海洋湖沼通报》2010年第3期49-55,共7页Transactions of Oceanology and Limnology

基　　金：教育部新世纪优秀人才支持计划资助(NCET-06-0597);高等学校创新引智计划(B08049)资助

摘　　要：研究了凡纳滨对虾血细胞的体外免疫活性保存技术。结果表明：4种保存液（CM、AC、MBHSS、M199）对凡纳滨对虾血细胞数量、存活率和酚氧化酶原级联系统胞吐影响显著（P〈0.05）,随着孵育时间的延长,4种保存液各处理组血细胞数量、存活率和丝氨酸蛋白酶原、酚氧化酶原活力逐渐下降,而酚氧化酶活力逐渐升高。在60min内CM处理组血细胞数量、存活率显著高于MBHSS和M199处理组,而CM与AC处理组在40min内无显著性差异,60min时CM处理组血细胞存活率明显高于AC处理组,而血细胞数量无显著差异;在0~20min内CM处理组丝氨酸蛋白酶原、酚氧化酶原活力均高于其它处理组,释放的酚氧化酶活力则低于其它处理组,而在40~60min内CM处理组与其它处理组各指标差异显著。同时在0~40min内CM处理组各指标无明显变化,60min时差异显著。由此可见,CM缓冲液可作为凡纳滨对虾血细胞体外免疫活性的保存液,而且体外实验应在40min内完成。In the paper,the suitable immune activity keeping method of Litopenaeus vannamei haemocyte was studied,and the effects of four preservative solutions（CM,AC,MBHSS,M199） on haemocyte counts,viability and exocytosis of prophenoloxidase cascade system were examined.It was founded that all four solutions have notable effects on haemocyte counts,viability,and exocytosis of prophenoloxidase（proPO） cascade system（P0.05）.During 60 minutes,the haemocyte counts,viability,serine proteinase and prophenoloxidase activity in four treatments declined gradually,while phenoloxidase activity climbed increasingly.However,the haemocyte counts,and viability in CM were significantly higher than those in MBHSS and M199,and there was no significant difference between CM and AC group during 40 min,cell viability in CM was higher than that in AC at 60 min.Both serine proteinase and prophenoloxidase activity in CM were higher than other treatments in the initial 20 min,while phenoloxidase activity was reversed.The parameters of CM group showed significantly different from those of other groups after 40 min.Meanwhile,every parameter in CM showed were no significant difference during 40 min,but obvious at 60 min.So,it is suggested that CM buffer can be chosen as a short-term preservative solution of Litopenaeus vannamei haemocyte,and the study in vitro should be completed in 40 min.

关 键 词：凡纳滨对虾 血细胞 免疫活性 保存技术 

分 类 号：S971.4[农业科学—捕捞与储运]