“锦绣”、“丰黄”黄桃变异株的SRAP分析  

SRAP analysis on variant lines of Jinxiu and Fenghuang yellow peach

作　　者：周平[1] 郭瑞[1] 雷龑[1] 金光[1] 廖汝玉[1] 陈小明[1] 黄新忠[1] 陈由强[2] 

机构地区：[1]福建省农业科学院果树研究所,福建福州350013 [2]福建师范大学生命科学学院,福建福州350108

出　　处：《福建农业学报》2010年第4期444-449,共6页Fujian Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD07B01-2);福建省科技计划重点项目(2007N0030,2008R0025);福建省公益类科研院所基本科研专项(2009R10029);福建省财政专项(STIF-Y06);福建省农业科学院青年科技人才创新基金(2008QA-5)

摘　　要：应用SRAP技术对"锦绣"黄桃、"丰黄"黄桃及其相应变异株系等7个材料进行了分析。从40对引物组合中筛选出2对引物在"丰黄"及变异株"锦黄"之间有明显扩增带差异,表明遗传变异已发生。分别纯化、克隆、测序这些特异条带,分析序列预测所示基因,并设计引物将其转化为稳定SCAR标记。应用特异的SCAR标记可快速检测鉴定相应变异株,进一步验证了变异的存在。试验未筛选到合适引物可区分"锦绣"及其变异株系。Seven yellow peach samples,including Jinxiu,Fenghuang and their variant lines,were studied by means of the sequence-related amplified polymorphism（SRAP） molecular marker technology.By screening a total of 40 SRAP primer combinations,it was found that 2 combinations could positively identify the differential amplification between Fenghuang and its variant line Jinhuang,as they were genetically diversified.Subsequently,these specific marker bands were cloned,sequenced,analyzed and converted into stable sequence characterized amplified region（SCAR） markers using the corresponding SCAR primers.The SCAR markers from SRAP were suitable for rapid identification of the variant lines.The evidence of the genetic diversity was further verified by SCAR PCR.On the other hand,DNA polymorphism between Jinxiu and its variant lines was not detected.

关 键 词：黄桃 变异株 相关序列扩增多态性 特征性片断扩增区域 

分 类 号：S662.1[农业科学—果树学]