SNAIL诱导MCF-7细胞上皮间质转化致阿霉素耐药性增加  

SNAIL-induced EMT enhances adriamycin-resistance capacity of MCR-7 cells

作　　者：王辉[1] 张义军[1] 陈伟娟[2] 李文通[2] 李莎莎[1] 丁保清[1] 

机构地区：[1]潍坊医学院药理学教研室,山东潍坊261041 [2]潍坊医学院病理学教研室,山东潍坊261041

出　　处：《基础医学与临床》2010年第10期1041-1045,共5页Basic and Clinical Medicine

基　　金：山东省自然科学基金(Y2008C75)

摘　　要：目的观察SNAIL诱导后MCF-7细胞上皮间质转化(EMT)的发生和P-糖蛋白(P-GP)的表达,探讨EMT致乳腺癌细胞耐药性增强的作用。方法构建SNAIL真核表达载体,转染MCF-7细胞,用免疫荧光检测上皮标记物E-钙黏素、间质标志物波形蛋白的表达;MTT法评价细胞对阿霉素(ADM)的耐药性;流式细胞术检测细胞中P-GP和SNAIL的表达;Real Time PCR检测SNAIL、E-钙黏素、波形蛋白、MDR1mRNA的表达。结果MCF-7细胞转染SNAIL后,E-钙黏素表达较亲本细胞显著降低,波形蛋白表达显著升高(P<0.01),对ADM的耐药性及SNAIL、P-GP的表达也显著升高(P<0.01),与Real Time PCR结果一致。结论SNAIL诱导MCF-7细胞EMT后,增强了乳腺癌细胞中P-GP介导的对ADM的耐药。Objective To observe the phenomenon of epithelial-mesenchymal transition（EMT） and expression of P-glucoprotein（P-GP） in MCF-7 cells,induced by SNAIL,and to find outcome of EMT enhancing drug-resistance of MCR-7 cells.Methods Constructed eukaryotic expression vector of SNAIL and then transfected into MCF-7 cells.Expression of epithelial marker E-cadherin and interstitial marker vimentin were detected by immunofluorescence;resistance to adriamycin（ADM） was measured by MTT;expression of P-GP and SNAIL was detected by flow cytometry;SNAIL,E-cadherin,Vimentin and MDR1 mRNA were evaluated by RT-PCR.Results Compared with parental MCF-7 cells,expression of E-cadherin decreased significantly,while vimentin got up（P0.01）;drug resistance to ADM and expression of SNAIL,P-GP increased too（P0.01）,and it was consistent with results of RT-PCR.Conclusion EMT,induced by SNAIL,can enhance the drug-resistance capacity to ADM,mediated by P-GP,in breast cancer cell.

关 键 词：乳腺癌 多药耐药 上皮间质转化 

分 类 号：R737.9[医药卫生—肿瘤]