腺苷抑制脂多糖诱导的人脐静脉内皮细胞凋亡  

Adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell apoptosis

作　　者：张传印[1] 张常娥[1] 秦爱萍[1] 卢晓玉[1] 赵冲[1] 陆丽[1] 

机构地区：[1]广州医学院病理生理学教研室,广东广州510182

出　　处：《基础医学与临床》2010年第10期1051-1054,共4页Basic and Clinical Medicine

基　　金：广东省自然科学基金(7003073)

摘　　要：目的探讨腺苷抑制脂多糖(LPS)诱导人脐静脉内皮细胞(HUVEC)的凋亡。方法将培养的HUVECs分成6组:对照组、二甲基亚砜(DMSO)组、LPS组、低、中和高剂量腺苷组,用荧光显微镜动态观察细胞形态结构,用ELISA检测上清液中TNF-α表达量,用流式细胞技术检测各组细胞凋亡。结果LPS组细胞凋亡率显著升高,TNF-α的表达量也显著升高(P<0.05);不同剂量腺苷能显著抑制LPS诱导的TNF-α表达,显著抑制细胞的凋亡率(P<0.05)。结论一定浓度的腺苷能抑制LPS诱导的HUVEC释放炎性介质,抑制细胞发生凋亡。Objective To explore the mechanism of adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell（HUVEC） apoptosis.Methods The cultured HUVECs were divided into 6 groups： control group,LPS group,dimethylsulfoxide（DMSO） group,low,medium and high-dose adenosine group.the cell＇s shape structure were continuously observed by fluorescence microscopy.TNF-α expression in the supernate was determined by ELISA.Cells＇s apoptosis was examined by flow cytometry.Results The TNF-α expression and cell apoptosis were significantly increased（P0.05）;Groups of different doses of adenosine could inhibit the expression of TNF-α and cell apoptosis（P0.05）.Conclusion A given dose of adenosine can inhibit lipopolysaccharide-induced the HUVEC release inflammation medium,adenosine can also suppress cell apoptosis.

关 键 词：腺苷 内毒素 人脐静脉内皮细胞 炎性反应 凋亡 

分 类 号：R363[医药卫生—病理学]