一氧化氮抑制胃癌细胞系SGC-7901增殖及C-FOS和C-JUN表达  

Nitric oxide inhibits cell proliferation and expression of C-FOS and C-JUN by gastric cancer cell line SGC-7901

作　　者：桑建荣[1] 陈永昌[1] 邵根宝[2] 黄晓佳[3] 

机构地区：[1]江苏大学基础医学与医学技术学院生理学系,江苏镇江212013 [2]江苏大学基础医学与医学技术学院生物学系,江苏镇江212013 [3]江苏大学基础医学与医学技术学院药理学系,江苏镇江212013

出　　处：《基础医学与临床》2010年第10期1072-1075,共4页Basic and Clinical Medicine

基　　金：江苏大学高级专业人才科研启动基金(08JDG033)

摘　　要：目的探讨一氧化氮(NO)对胃癌细胞系SGC-7901增殖的影响及其机制。方法MTT法测定细胞的生长,RT-PCR和W estern印迹法检测C-FOS和C-JUN的mRNA和蛋白表达。结果硝普钠(SNP)抑制SGC-7901细胞的生长(P<0.05),并呈剂量和时间性降低C-FOS和C-JUNmRNA及蛋白表达。结论NO可能通过下调C-FOS和C-JUN的表达而抑制胃癌细胞的增殖。Objective To investigate the effect of nitric oxide（NO） on cell proliferation of gastric cancer cell line SGC-7901,and to investigate the relevant mechanisms.Methods MTT assay was used to detect the inhibitory effect of NO on cell growth.RT-PCR was used to detect the mRNA expression of C-FOS and C-JUN.Western blot was applied to study the protein expression of C-FOS and C-JUN.Results Sodium nitroprusside（SNP） inhibited the proliferation of SGC-7901 cells（P0.05）.SNP dose-and time-dependently decreased the expression of C-FOS,C-JUN mRNA and protein.Conclusion NO inhibits the proliferation of SGC-7901 cells,which is probably down-regulated C-FOS and C-JUN expression through NO.

关 键 词：胃肿瘤 一氧化氮 SGC-7901细胞系 

分 类 号：R735.2[医药卫生—肿瘤]