新疆荒漠束颈蝗属及其近、远缘种的线粒体DNA RFLP与系统进化  

PHYLOGENETIC RELATIONSHIP AMONG NINE SPECIES OF SPHINGONOTUS FIEBER IN DESERT AREA OF XINJIANG INFERRED FROM MITOCHONDRIAL DNA VARIATIONS

作　　者：邵红光[1] 严健[1] 庚镇城[1] 李国强[2] 黄人鑫[2] 

机构地区：[1]复旦大学遗传学研究所 [2]新疆大学生物学系

出　　处：《昆虫学报》1999年第2期132-140,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金

摘　　要：以１１种限制性内切酶对新疆荒漠中的９种束颈蝗和与其近缘的细距蝗Ｌｅｐｔｏｐｔｅｒｎｉｓｇｒａｃｉｌｉｓ，旋跳蝗Ｈｅｌｉｏｓｃｉｒｔｕｓｍｏｓｅｒｉｍｏｓｅｒｉ及远缘的意大利蝗Ｃａｌｉｐｔａｍｕｓｉｔａｌｉｃｕｓｉｔａｌｉｃｕｓ，红翅瘤蝗Ｄｅｒｉｃｏｒｙｓｒｏｓｅｉｐｅｎｎｉｓ的线粒体ＤＮＡ进行了长度片段多态性的研究。根据所得的酶切类型，计算了种间每核苷酸位点的平均碱基取代值Ｐ（遗传距离）。用ＵＰＧＭＡ法构建分子系统树。结果表明，束颈蝗属种间的Ｐ值约为００９９～０１４６，与近缘种属间约０１００～０２０５，与远缘种属间约为０１１３～０２０６。估算出束颈蝗属种间的分歧年代约在１０００～１５００万年前，处于中新世中期，将实验结果与形态演化比较分析。In this study, 11 restriction endonucleases were used to analyze the RFLP of mitochondrial DNA purified from nine species of Sphingonotus Fieber, Leptopternis gracilis which has close relationship with Sphingonotus and Calliptamus italicus which has somewhat distant relationship with Sphingonotus. We estimated the average number of nucleotide substitutions per site (genetic distance, P ) among these species according to the cleavage patterns and Nei & Li's models(1979). We also constructed the molecular phylogenetic trees and studied the phylogenetic relationship by the UPGMA method . These results show that the P values among the nine species of Sphingonotus are about 0 099 to 0 146, the P values between Sphingonotus and the distant genus are about 0 113～0 226. So the divergence time among the species of Sphingonotus would be about 10～15 million years.

关 键 词：束颈蝗属 线粒体 DNA变异 系统进化 

分 类 号：Q969.265.1[生物学—昆虫学]