Tet调控的EB病毒LMP1基因导入鼻咽癌细胞系表达的研究  

Doxycycline Dependent Expression of Epstein Barr Virus Latent Membrane Protein 1 with Tet Regulating System in Nasopharygeal Carcinoma Cell Line

作　　者：廖伟[1] 易红[1] 李晓艳[1] 唐敏[1] 顾焕华[1] 曹亚[1] 

机构地区：[1]湖南医科大学肿瘤研究所

出　　处：《生物化学与生物物理学报》1999年第3期309-312,共4页

基　　金：国家自然科学基金;国家自然科学杰出青年基金;中华医学基金

摘　　要：本文利用Ｔｅｔｏｎ基因表达系统建立了一株可诱导ＥＢ病毒ＬＭＰ１基因表达的鼻咽癌细胞株。首先将Ｔｅｔｏｎ基因调控系统的调节质粒ｐＴｅｔｏｎ导入鼻咽癌细胞系ＨＮＥ２中，用ｒｔＴＡ反应性荧光素酶报道基因ｐＴＲＥｌｕｃ挑选高诱导、低背景的克隆，第二轮转染将构建的反应质粒ｐＴＲＥＬＭＰ１导入得到稳定转染的阳性克隆，对各克隆用Ｗｅｓｔｅｒｎ印迹方法进行强力霉素（Ｄｏｘ）诱导效应检测，其中Ｌ７对Ｄｏｘ具有较好的剂量反应依赖性，诱导表达量可增加１０倍左右。ＬＭＰ１在鼻咽癌细胞可调表达对于分析其不同程度的表达在鼻咽癌发生中的作用具有重要价值。Latent membrane protein 1(LMP1) of Epstein Barr virus (EBV) plays a vital role in cell transformation and the nasopharygeal carcinoma (NPC) is highly related to EBV infection. To elucidate whether LMP1 exerts oncogenic effect in NPC and its possible mechanism, a dual stable LMP 1 integrated NPC cell line with Tet on regulating system were established. Results revealed that expression of LMP 1 could be regulated by Tet system when NPC cell line HNE 2 served as receipt cell, and doxycycline could increase this expression by about 10 fold. Therefore, it is possible that different level expression of LMP 1 results in its different biological functions and various efforts on nasopharyngeal carcinogenesis.

关 键 词：EB病毒 潜伏膜蛋白 基因 诱导表达 鼻咽癌 

分 类 号：R739.630.2[医药卫生—肿瘤] R373.9[医药卫生—临床医学]