纳洛酮对少突胶质细胞的保护作用  

Protective Effect of Naloxone on Oligodendrocytes Injury Caused by LPS Activated Microglial Cells

作　　者：王银[1,2] 李云鸿[1] 滕鹏[2] 苗珍花[2] 杨文君[1,3] 李博[2] 

机构地区：[1]宁夏医科大学科学技术中心,银川750004 [2]宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川750004 [3]宁夏医科大学基础医学院生物技术系,银川750004

出　　处：《宁夏医科大学学报》2010年第6期654-656,F0002,共4页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(30960108);宁夏医科大学特殊人才项目(XT200910)

摘　　要：目的研究纳洛酮(naloxone)对脂多糖(lipopolysacchar,LPS)激活的小胶质细胞诱导少突胶质细胞损伤的影响。方法共培养大鼠小胶质细胞和少突胶质细胞,用LPS(1μg.mL-1)刺激小胶质细胞,随后用0.1μM纳洛酮进行干预,应用免疫组化方法观察计数少突胶质细胞。结果仅用0.1μM纳洛酮处理细胞,细胞活性基本保持不变,而当小胶质细胞被LPS激活后,与之共培养的大量的少突胶质细胞死亡(P<0.01);小胶质细胞被LPS激活,给予0.1μM纳洛酮处理后,少突胶质细胞的活细胞数显著升高(P<0.05)。结论纳洛酮能够抑制LPS激活的小胶质细胞引起的少突胶质细胞的损伤。Objective To explore the protective effect of naloxone on oligodendrocytes injury caused by LPS activated microglia cells.Methods Microglia cells cocultured with oligodendrocytes were treated with LPS（1μg·mL-1）,then treated with 0.1μM naloxone for 18 h.The protective effect of naloxone was measured by IHC staining.Results After microglia cells were activated by LPS,the injury of cocultured oligodendrocytes could be obviously shown.However,treatment of LPS activated microglia cells with 0.1μM naloxone significantly prevented against the oligodendrocytes injury caused by microglia activation.Conclusion Although naloxone can protect oligodendrocytes injury from activated microglia cells,it would be a good candidate for treating some neurodegenerative diseases.

关 键 词：纳洛酮 少突胶质细胞 小胶质细胞 LPS 

分 类 号：R741.02[医药卫生—神经病学与精神病学]