新生大鼠缺氧缺血再灌注脑损伤肾上腺髓质素表达的研究  

Adrenomedullin expression in newborn rats with hypoxic-ischemic reperfusion brain damage

作　　者：张士发[1] 周名雄[1] 茅双根[1] 窦常胜[1] 严颜[1] 李志奇[1] 

机构地区：[1]皖南医学院附属弋矶山医院儿科,安徽芜湖241001

出　　处：《皖南医学院学报》2010年第5期321-323,330,共4页Journal of Wannan Medical College

基　　金：安徽省高等学校省级自然科学研究项目(2006KJ403B)

摘　　要：目的：探讨新生大鼠缺氧缺血再灌注脑损伤（hy-poxic-ischemic reperfusion brain damage,HIRBD）后不同时间脑组织肾上腺髓质素（Adrenomedullin,AM）mRNA、AM及外周血血浆AM水平变化规律。方法：将42只健康7 d龄SD实验大鼠随机分为正常对照组、假手术组、HIRBD后0 h、6h、12 h、24 h、48 h组,每组6只,按时剖杀各组实验大鼠,断头取血及双侧脑组织,采用半定量RT-PCR法测定脑组织AM mRNA,酶联免疫吸附法测定脑组织和血浆AM水平。结果：①HIRBD各组脑组织AM mRNA、AM和血浆AM水平显著性增高,与对照组比较有统计学意义（P均〈0.05）,高峰在6~24 h,24 h后开始下降。②脑组织AM mRNA水平和脑组织AM水平呈正相关（r=0.75,P〈0.05）。③外周血血浆AM水平和脑组织AM水平呈正相关（r=0.78,P〈0.05）。结论：缺氧缺血再灌注可诱导新生大鼠脑组织AM基因表达和转录水平的增高,并具有一定的时间依赖性;新生大鼠HIRBD时脑组织AM基因表达和转录水平的增高可能是外周血血浆AM增高的主要原因。Objective：To investigate the mRNA expression of adrenomedullin（AM） in cerebral tissue at different time and variation of AM and plasma AM in neonatal rat model with hypoxic-ischemic reperfusion brain damage（HIRBD）.Methods： Forty-two Sprague-Dawley（SD） rats aged 7d were randomized into groups of normal control,sham operation and HIRBD at 0 h,6 h,12 h,24 h or 48 h,respectively（n=6 for each group）,and were decapitated for obtaining the blood and brain tissues.Semi-quantitative RT-PCR method was used to determine the mRNA expression of AM in the cerebral tissue and enzyme-linked immunosorbent assay（ELISA）,for measuring the levels of AM in cerebral tissue and AM.Results： ①The expression of AM mRNA and the levels of AM in plasma and cerebral tissue were increased significantly in each group,with peak at between 6 h and 24 h,but fall after 24 h,as compared with the controls and sham operation group（P0.05）;②The expression of AM mRNA was positively correlated with level of AM in the cerebral tissue（r=0.75,P0.05）;③The levels of cerebral tissue AM were also positively correlated with plasma AM（r=0.78,P0.05）.Conclusion： Hypoxic-ischemic reperfusion may result in up-regulated mRNA expression and transcription of AM in cerebral tissue of neonatal rats,with certain time-dependence,and the increased mRNA expression and level of AM in the experimental animals with HIRBD appears associated with elevated plasma AM level.

关 键 词：新生大鼠 肾上腺髓质素 缺氧缺血 再灌注 

分 类 号：R743.31[医药卫生—神经病学与精神病学]