瞬时表达比较2种不同的RNAi载体的干涉效果  

Comparision of resistance to Potato virus Y(PVY) of two different RNAi vectors via transient expression

作　　者：邱礽[1,2] 陶刚[2,3] 邱又彬[1,2] 李奇科[1,2] 朱英[2,4] 迟胜起[5] 刘作易[2] 

机构地区：[1]贵州大学生命科学院,贵阳550025 [2]贵州省农业生物技术重点实验室,贵阳550006 [3]贵州省植物保护研究所,贵阳550006 [4]贵州省生物技术研究所,贵阳550006 [5]青岛农业大学植物保护学院,青岛266109

出　　处：《植物病理学报》2010年第5期543-547,共5页Acta Phytopathologica Sinica

基　　金："973"计划前期研究专项(2008CB117010);贵州省科学技术重大专项[黔科合重大专项字(2008)6009号];贵州省农业科学院重点专项项目[院2X(2007)002号]

摘　　要：RNAi with natural defence mechanism of homologous RNA degradation is widely used in research of antiviral plant.It is important to construct a highly efficent RNAi vector for transgenic plants of virus resis-tance.In this study,part fragments of coat protein gene of Potato virus Y(PVY)(451-750 bp) were inserted into the two expression vectors.Vector pROKY300 without intron and pHelY300 with PDK and CAT introns on the hpRNA stem were constructed.The silence efficiency of virus resistance of the two vectors was investigated as 88%(22/25)for pROKY300 and 92%(23/25) for pHelY300 through transient expression mediated by agroinfiltration.The results showed that both vectors were highly antiviral and elucidated the validity of RNAi-medicates resistance to virus.RNAi with natural defence mechanism of homologous RNA degradation is widely used in research of antiviral plant.It is important to construct a highly efficent RNAi vector for transgenic plants of virus resis-tance.In this study,part fragments of coat protein gene of Potato virus Y（PVY）（451-750 bp） were inserted into the two expression vectors.Vector pROKY300 without intron and pHelY300 with PDK and CAT introns on the hpRNA stem were constructed.The silence efficiency of virus resistance of the two vectors was investigated as 88%（22/25）for pROKY300 and 92%（23/25） for pHelY300 through transient expression mediated by agroinfiltration.The results showed that both vectors were highly antiviral and elucidated the validity of RNAi-medicates resistance to virus.

关 键 词：RNAI载体 瞬时表达 马铃薯Y病毒 植物表达载体 干涉 反向重复序列 抗病毒 复合侵染 

分 类 号：S432.41[农业科学—植物病理学]