姜黄素对肿瘤细胞中β3Gn-T8及其相关基因表达的影响  

Study on the Effect of Curcumin to the β3Gn-T8 and Its Related Genes in Tumor Cell Lines

作　　者：周进伟[1] 沈力[1] 姜智[1] 徐岚[1] 吴士良[1] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院生物化学与分子生物学教研室,江苏苏州215123

出　　处：《中国血液流变学杂志》2010年第3期349-352,共4页Chinese Journal of Hemorheology

基　　金：国家自然科学基金资助项目（30670462）

摘　　要：目的 以不同浓度的姜黄素处理U253,SMMC-7721细胞,从mRNA水平观察GnT8、GnT2、MMP2表达的变化.方法 以不同浓度的姜黄素处理细胞,24h、48h后RT-PCR检测GnT8、GnT2、MMP2的表达.结果 随着药物浓度、时间的增加,MMP2、GnT8表达明显降低,同时GnT2表达降低,但降低的程度较轻.结论 姜黄素可能通过下调肿瘤细胞中GnT8、GnT2的表达改变了细胞表面的糖链结构,而该改变可能参与了姜黄素诱导的肿瘤细胞凋亡.Objective To study the variation of β3Gn-T8 and its related genes expression in U251 and SMMC-7721 celt lines after curcumin added to the culture medium. Methods Using RT-PCR to detect（l） the mRNA expression of MMP2,GnT2,GnT8 in U251 cell line and SMMC-7721 cell line;（2）the expression discrepancy of GriT2 and GriT8 in U251 cell line and SMMC-7721 cell line after curcumin added to the culture medium. Results After curcumin added to the culture medium,the mRNA expressions of GnT2,GnT8,and MMP2 in U251 and SMMC-7721 cell line decreased, and mRNA expressions of MMP2 decreased strongly. Conclusion This study showed that the expression of GnT2 and GnT8 are different in the two cell line after curcumin added to the culture medium.This result suggested that the effect of curcumin may change the sugar chains of glycoprotein in cell surface.

关 键 词：姜黄素 β3Gn-T8 MMP2 

分 类 号：R73-3[医药卫生—肿瘤]