同病异证和异病同证的物质基础——肿瘤、高血压、糖尿病大鼠/小鼠神经-内分泌组织一些重要激素的转录特征  

Material Basis of "Same Disease with Different Syndromes" and "Different Diseases with Same Syndrome"——Gene Transcription Characteristics of Some Important Hormones in Neuroendocrine Tissues of Rats/Mice with Tumor,Hypertension or Diabetes Mellitus

作　　者：方肇勤[1] 潘志强[1] 卢文丽[1] 刘小美[1] 管冬元[1] 梁超[1] 高必峰[2] 

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]美国科罗拉多大学医学院,丹佛co80262

出　　处：《上海中医药大学学报》2010年第5期58-67,共10页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市科委国际合作项目(064307052);上海市教委学科建设项目(2006)

摘　　要：目的:揭示肿瘤、高血压、糖尿病同病异证、异病同证大鼠、小鼠神经-内分泌组织一些重要激素表达的特征。方法:采用腋下接种H22荷瘤小鼠,辨证出早期邪毒壅盛证和气虚证、中期阳气虚证和气虚证、中晚期气阴阳虚证和气虚证;采用自发性高血压大鼠和自发性糖尿病大鼠,辨证出气盛证和气虚证;分别与正常和正常气虚证大鼠、小鼠对照;采用Affymetrix外显子芯片,检测其下丘脑、垂体、甲状腺、肾上腺、性腺等组织基因表达的差异;重点观察CRH、POMC,TRH、TSH,GnRH、FSH、LH,GhRH、GH等基因表达的改变。结果:①大鼠、小鼠下丘脑TRH表达量较CRH、GnRH、GhRH大;垂体TSH、POMC、FSH、GH表达量大,并不同程度表达GnRH、GhRH、CRH;甲状腺、肾上腺、性腺等亦不同程度地表达POMC、TSH、FSH、LH、GH,小鼠尤甚,但大多没有规律;正常大鼠睾丸高表达LH。②正常大鼠、小鼠气虚证者,这些激素水平不同程度下调,唯小鼠TRH和TSHb上调。③疾病发生后,对机体危害程度较重的证候,如肿瘤的邪毒壅盛证、高血压的气盛证,其神经-内分泌有关激素的表达抑制程度略重于同期的气虚证。结论:大鼠、小鼠神经-内分泌一些重要激素调节模式略有差异。总体上,气虚证大鼠、小鼠神经-内分泌轴轻度抑制,疾病危重者抑制更显著,小鼠甲状腺轴例外。Objective：To study the gene expression characteristics of some important hormones in neuroendocrine tissues of tumor mice and spontaneously hypertensive rats（SHRs） and Goto-Kakisaki （GK） Wistar rats. Methods： The pathogenic toxin-exuberance syndrome and qi-deficiency syndrome in early stage,yang-qi deficiency syndrome and qi deficiency syndrome in intermediate stage,qi-yin-yang deficiency syndrome and qi deficiency syndrome in advanced stage were differentiated in H22 tumor-bearing mice; the qi excess syndrome and qi deficiency syndrome were differentiated in SHRs and GK rats; these model rats and mice were compared with normal and normal with qi deficiency syndrome rats and mice respectively; the gene expressions in hypothalamus,pituitary gland,thyroid gland,adrenal gland and gonad were detected by Affymetrix GeneChip; the gene expressions of CRH,POMC,TRH,TSH,GnRH,FSH,LH,GhRH and GH were observed. Results： （1）The expression of TRH in hypothalamus of rats and mice was higher than CRH,GnRH and GhRH; the expressions of TSH,POMC,FSH and GH in pituitary gland were higher and the GnRH,GhRH and CRH were also expressed at different degrees; the POMC,TSH,FSH,LH and GH were expressed in thyroid gland,adrenal gland and gonad at different degrees especially in mice,but there was no regularity; the expression of LH in spermary was higher in normal rats. （2）The levels of these hormones were lower in normal rats and mice with qi deficiency,while the expressions of TRH and TSHb were higher in mice. （3）The inhibitory degree of expressions of neuroendocrine hormones was more severe in the syndromes with severe harm on body such as pathogenic toxin-exuberance syndrome of tumor and qi excess syndrome of hypertension than those in qi deficiency syndrome at the same stage after pothogenesy. Conclusion：There is differentiation on regulating mode of some important hormones in neuroendocrine tissues of rats and mice. As a whole,the neuroendocrine axis is inhibited lightly in rats and mice with qi deficiency s

关 键 词：中医辨证 肿瘤 高血压 糖尿病 神经-内分泌 激素 基因表达 

分 类 号：R228[医药卫生—中医基础理论]