受体血清注入前房湿房保存角膜的实验研究  

Experiment study on corneal moist chamber storage with aqueous removed and recipient serum tamponaded

作　　者：胡庆军 邓青[1] 周文天[2] 

机构地区：[1]民航总医院眼科,北京100123 [2]南昌大学第二附属医院眼科

出　　处：《中国医师杂志》2010年第9期1183-1186,共4页Journal of Chinese Physician

摘　　要：目的 评价受体血清填充前房湿房保存角膜的角膜内皮细胞活性,以探讨一种新的角膜保存方法.方法 40只新西兰白兔的80只眼随机分2组：A组（对照组）40只眼和B组（实验组）40只眼.A组以传统的湿房保存法4℃保存角膜,B组以前房抽空房水注入受体血清湿房保存角膜.分别于保存后2、5、7、10 d行扫描电镜观察超微结构及锥虫蓝-茜素红检测角膜内皮细胞活性;角膜厚度仪测量角膜厚度及图像分析系统计算内皮细胞密度和细胞面积.结果 B组保存的角膜7 d内透明,内皮细胞活性大于90%,保存10d的角膜内皮细胞活性率大于80%;A组保存的角膜2 d内透明,保存5 d的角膜内皮细胞均溶解、死亡.B组保存7 d与A组保存2 d的角膜内皮细胞活性、内皮细胞密度、内皮细胞面积差异无统计学意义（P＞0.05）.A组保存5 d的角膜厚度（0.64±0.04）mm,保存7 d的角膜厚度（0.79±0.03）mm,保存10 d的角膜厚度（1.06±0.03）mm,B组保存5 d的角膜厚度（0.55±0.03）mm,保存7 d的角膜厚度（0.65±0.02）mm,保存10 d的角膜厚度（0.85±0.05）mm,两组比较差异有统计学意义（P＜0.05）.结论 受体血清有维护角膜内皮细胞结构和功能的作用,湿房保存的同时延长了角膜保存的时间.Objective To explore the new preservation method of cornea by evaluating the structure and function of rabbit＇s endothelia on the condition of whole-eye preservation with aqueous removed and recipient＇s serum tamponaded. Methods Forty New Zealand big white rabbits （80 eyes） were random divided into two groups, 40 eyes in group A （control group） and 40 eyes in group B （experimental group）. The vitality of endothelial cell on the condition of tow corneal preservation methods which were the moist chamber preservation （group A） and the whole-eye preservation with aqueous removed and recipient＇s serum tamponaded （group B） was compared. At the 2nd day, the 5th day, the 7th day, the 10th day, the vitality of endothelial cell was appraised through ultra-microstructure by scanning electron microscope and the trpan biuealizarin red stain. The corneal thickness was measured, and corneal endothelial cells density was calculated, and cell size was observed by image analysis system. Result In group B, the corneas remained transparent for 7 days , and the rate of vitality was 90% for7 days and that was over 80% for 10 days. In group A, the corneas remained transparent for 2 days, and the endothelial cell losing and dying were found after 5 days. Cell vitality, cell density and cell size had no statistical difference between Group B for 7 days and group A for 2 days. （all P〉0. 05）. In group A, at the 5th day, the 7th day ,the 10th day, the average corneal thickness were （0.64 ± 0.04） mm, （0. 79 ± 0. 03） mm , （1.06 ± 0. 03） mm. In the group B, at the 5th day, the 7th day ,the l0th day, the average corneal thickness were （0. 55 ±0.03）mm, （0.65 ±0. 02） mm , （0. 85 ± 0. 05） mm. The average corneal thickness had significant difference between group A and group B （all P 〈 0. 05）. Conclusion Recipient serum had the function with supporting the structure and function of rabbit＇s corneal endothelial cell, and it could prolong the storage time with the moist chamberstor

关 键 词：免疫血清/投药和剂量 内皮 角膜/细胞学 前房 组织保存 

分 类 号：R77[医药卫生—眼科]