精胺普鲁兰糖对荧光素钠角膜通透性的影响  

Effect of sperminated pullulans on transcorneal permeability of fluorescein sodium

作　　者：于楠楠[1] 金迪[1] 杨洪滨[1] 张丽琼[1] 刘洁[1] 崔浩[1] 

机构地区：[1]哈尔滨医科大学附属第一医院眼科,150001

出　　处：《眼科研究》2010年第10期917-920,共4页Chinese Ophthalmic Research

摘　　要：目的研究不同氨基含量的精胺普鲁兰糖(SP)对荧光素钠角膜渗透作用的影响,并评估其眼部刺激性。方法 9只家兔随机分为未加入SP的对照组、低氨基含量精胺普鲁兰糖(SP-L)组和高氨基含量精胺普鲁兰糖(SP-H)组,每组3只兔6只角膜。收集新鲜的离体角膜采用上皮面朝向Franz扩散池的供给池、内皮面朝向接受池的方法进行固定,采用离体角膜扩散实验,分别加入质量分数0.2%SP-L(氨基含量0.124mmol/g)或0.2%SP-H(氨基含量0.578mmol/g),用高效液相色谱仪测定30、60、90、120、150、180、210、240min样品中的荧光素钠质量浓度,采用干燥称重法测量各组的角膜水化值,并进行改良的Draize试验评分以评估SP对眼组织的刺激性。结果与对照组相比,SP-L组和SP-H组荧光素钠的表观渗透系数分别增加了2.12倍和6.87倍,SP-L组和SP-H组荧光素钠对角膜上皮的渗透参数明显高于对照组,差异均有统计学意义(P<0.05,P<0.01);SP-H组荧光素钠对角膜上皮的渗透参数明显高于SP-L组,差异有统计学意义(P<0.05)。3组角膜水化值均小于83%,差异无统计学意义(F=2.650,P=0.130)。3组改良Draize试验评分均小于4,提示SP应用后无眼部刺激作用。结论 SP是一种安全、有效的渗透促进剂,能够提高水溶性药物的角膜通透性,且随氨基含量的增多促渗透作用增强。Background Cationic polymers have great potential in improving the permeability through cornea of eye drug.Sperminated derivations are new generation of cationic polymers.Objective This study intended to evaluate the effect of sperminated pullulans （SP） on transcorneal permeability of fluorescein sodium and examine the relationship between the amino groups and the enhancement in vitro.The ocular irritation of SP was also tested both in vivo and in vitro.Methods A total of 9 clean New Zealand white rabbits were randomly divided into control group,Sperminated pullulans（SP）-L group and SP-H group and 6 corneas were included in each group.The freshly excised corneas were immediately mounted in modified Franz perfusion cells under the mixing condition with the corneal epithelium toward the deliver chamber and the endothelium toward the receptor chamber.Fluorescein sodium solution containing free-SP,0.2% SP with 0.124 mmol/g amino （SP-L） or 0.2% SP with 0.578 mmol/g amino （SP-H） was added into the deliver chamber respectively in each group.At 30,60,90,120,150,180,210,240 minutes after addition of fluorescein sodium solution,100 μL of the receptor solution was removed.The concentration of fluorescein sodium was determined by high pressure liquid chromatography.Hydration level of cornea was calculated at the end of each permeation run by the usual gravimetric method.A modified Draize test was carried out to evaluate the eye irritation in vivo.Results The apparent permeability coefficient （Papp） for fluorescein sodium increased by 2.12 fold in SP-L group and 6.87 fold in SP-H group.Fluorescein sodium transcorneal permeation parameters were significantly enhanced in SP-L and SP-H in comparison with control group （P0.05,P0.01）,respectively,and that in SP-H group was the more effective enhancer than SP-L group （P0.05）.The corneal hydration value was less than 83%.No evident difference was found in corneal hydration value among control group,SP-L group and SP-H group （F=2.650,P=0.130）.The scor

关 键 词：精胺普鲁兰糖 荧光素钠 渗透促进剂 角膜 

分 类 号：R772[医药卫生—眼科] R988.1[医药卫生—临床医学]