NK细胞杀伤人胶质瘤起始细胞的体外研究  

Studies on Cytotoxicity of Natural Killer (NK) Cells against Glioma Initiating Cells in Vitro

作　　者：史泓浏[1] 付军[1] 陈芙蓉[1] 陈忠平[1] 

机构地区：[1]中山大学肿瘤防治中心神经外科/神经肿瘤科,广东广州510060

出　　处：《中国神经肿瘤杂志》2009年第4期227-230,共4页Chinese Journal of Neuro-Oncology

基　　金：国家自然科学基金(Νο.30772551)

摘　　要：背景与目的:越来越多的证据支持人脑胶质瘤来源于胶质瘤起始细胞/干细胞(glioma initiating cell/stem cell,GIC/GSC)的假说,鉴于干细胞特性,常规手术和放化疗难以清除GIC,本研究试图用体外活化的自然杀伤(natural killer,NK)细胞对GIC细胞进行杀伤研究。方法:以体外长期培养于干细胞培养基的CD133阳性胶质瘤细胞作为GIC,用免疫磁珠从胶质瘤患者(或健康人)的外周血中分离NK细胞,常规IL2/PHA活化NK细胞后,应用LDH释放法检测其体外杀伤GIC细胞的活性,以K562细胞为阳性对照。结果:体外实验中,异体NK细胞可以在GIC的培养基里生长和活化,且其杀伤GIC细胞的能力随效靶比增高而增强,同一效靶比时,活化的NK细胞杀伤GIC细胞的活性明显高于未活化(静止)NK细胞的活性(P<0.01)。结论:异体NK细胞可以在GIC的培养环境中活化并表现出针对GIC的杀伤能力,为NK细胞用于脑胶质瘤起始细胞的临床清除提供可能性。BACKGROUND ＆ OBJECTIVE：There are increasing evidence to support the hypothesis that human gliomas originated fi＇om glioma initiating cells or stem cells （GIC/GSC）. These cells can not be eradicated by conventional surgeu, chemotherapy and radiotherapy because of their ＂stem-like＂ properties. In this study, the cytotoxicity of activated natural killer （NK） cells against GIC in vitro was investigated. METHODS ： NK cells were isolated from peripheral blood monormelear cells of glioma patients or heahhy donors by Miltenyi magnetic beads, and were activated with IL2/PHA. Their in vitro cytotoxicity against GIC was assessed by lactate dehydrogenase （LDH） assay. RESULT： The allogeneic NK cells were vital and cotdd be activated with GIC-cuhured medium. Increased cytolytie activity against GIC was obsep,ed with the higher E：T ratio. At the same E：T ratio, activated NK cells showed remarkable higher cytolytic activity against GIC than that of resting NK cells. CONCLUSIONS ： Allogeneic NK cells demonstrate killing effect against GIC. It is suggested that activated NK cells may be promising to eradicate GIC.

关 键 词：活化NK细胞 胶质瘤起始细胞/干细胞 细胞杀伤 LDH实验 

分 类 号：R739.41[医药卫生—肿瘤]