全脑缺血再灌注兴奋性氨基酸、线粒体钙、钙调素含量的变化  

Experimental study on changes of hippocampus EAA,MCa,CaM contents on rat global cerebral ischemic reperfusion damage

作　　者：徐仁伵 刘煜敏[1,2,3,4] 赵宏选 黄怀钧 孙圣刚[1,2,3,4] 李志成[1,2,3,4] 

机构地区：[1]武汉同济医科大学协和医院神经科 [2]湖北医科大学第二附属医院神经科 [3]河南省禹州市卫校药剂科 [4]江西省弋阳港口医院

出　　处：《卒中与神经疾病》1999年第2期92-93,共2页Stroke and Nervous Diseases

摘　　要：目的：采用大鼠全脑缺血再灌注模型，观察海马组织兴奋性氨基酸、线粒体钙、钙调素含量的变化，研究分析脑缺血再灌注损伤中兴奋性氨基酸与钙平衡紊乱的变化和作用。方法：测定假手术组，缺血３０ｍｉｎ再灌注６０ｍｉｎ和缺血３０ｍｉｎ再灌注１２ｈ组，脑海马组织兴奋性氨基酸、线粒体钙、钙调素的含量。结果：缺血３０ｍｉｎ再灌注６０ｍｉｎ海马组织兴奋性氨基酸明显低于假手术组（Ｐ＜０．０１），线粒体钙、钙调素含量显著性高于假手术组（Ｐ＜０．０１），缺血３０ｍｉｎ再灌注１２ｈ组同假手术组比较没有显著性差异（Ｐ＞０．０５）。结论：我们从鼠脑缺血再灌注时线粒体钙。Objective :In order to study the relation between excitatory amino acid (EAA),calcium abnormal balance and cerebral ischemic reperfusion damage,the changes of rat hippocampus EAA,mitochondria calcium (MCa),calmodulin (CaM) were observed on cerebral ischemic reperfusion model (4VO). Methods :We measured respectively hippocampus glutamate (Glu),aspartate (Asp),MCa,CaM contents on sham operated group,ischemia 30 min reperfusion 60 min and ischemia 30 min reperfusion 12h group. Results :We found that the EAA contents significantly decreased and the MCa,CaM contents significantly increased in ischemia 30 min reperfusion 60 min group,the EAA,MCa,CaM contents nearly came back to control's,the change of EAA contents had cooperative relation to that of MCa,CaM contents during ischemic reperfusion damage. Conclusion :According to changes of EAA,MCa,CaM,we suggested that calcium abnormal balance play a important role in ischemic reperfusion cerebral damage produced by EAA.

关 键 词：脑缺血 再灌注损伤 兴奋性氨基酸 线粒体钙 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]