马鞭草裂环环烯醚萜苷类成分的分离与鉴定  

Isolation and identification of chemical constituents of secoiridoid glycosides from the aerial parts of Verbena officinalis L.

作　　者：徐伟[1] 辛菲[1] 刘明[1] 沙沂[1] 方瑾[2] 李玉山[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]中国医科大学基础医学院,辽宁沈阳110001

出　　处：《沈阳药科大学学报》2010年第10期793-796,共4页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省教育厅科研基金资助项目(2004C073)

摘　　要：目的研究马鞭草中裂环环烯醚萜苷类成分。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱和半制备高效液相色谱等方法进行分离纯化，依据MS、1D和2DNMR（^1H-HCOSY、HMQC和HMBC）进行结构鉴定。结果从马鞭草乙醇提取物的正丁醇可溶部分分离得到5个裂环环烯醚萜苷类化合物，分别鉴定为三叶草苷（trifloroside，1）、macrophylloside（2）、3’-acetyl—swemside（3）、当药苦苷（swertiamarin，4）和龙胆苦苷（gentiopicmside，5）。结论化合物1—4为首次从马鞭草属植物中分离得到。Objective To study the secoiridoid glycoside constituents of Verbena officinalis L. Methods The compounds were isolated by silica gel, Sephadex LH-20 and semiprep. HPLC column chromatography and MS,1D and 2D NMR（ ^1H-^1H COSY,HMQC and HMBC）were employed for the structural identification. Results Compounds 1- 5 were obtained from the n-BuOH soluble constituents of Verbena officinalis and their structures were identified as trifloroside （ 1 ）, macrophylloside （ 2 ）, 3＇-acetylsweroside （ 3 ）, swertiamarin （4） and gentiopicroside （5）, which are secoiridoid glycosides. Conclusions Compounds 1-4 are isolated from the genus Verbena for the first time.

关 键 词：马鞭草 裂环环烯醚萜苷类 化学成分 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R284[医药卫生—药学]