Liquid Chromatographic Assay of Peptides Activity with Inhibiting Angiotensin Converting Enzyme  

作　　者：LIU Jing-bo YU Zhi-peng ZHAO Wen-zhu LU Jing CHEN Feng LIN Song-yi 

机构地区：[1]Laboratory of Nutrition and Functional Food, Jilin University, Changehun 130062, P. R. China [2]Department of Food Science and Human Nutrition, Clemson University, Clemson SC 29634, USA

出　　处：《Chemical Research in Chinese Universities》2010年第5期712-716,共5页高等学校化学研究（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(No.2007AA10Z329)

摘　　要：A rapid, simple and interference-free method was developed to evaluate the inhibitory activity of hydrolyzed peptides from egg white protein against the angiotensin-converting enzyme. The total reaction volume was 60 μL, saving the cost. The assay was based on a HPLC separation and quantification of the synthetic substrate hippuryl-L-histidyl-L-leucine and its hydrolyzed product-hippuric acid; the separation was performed on a C18 column eluted by a mobile phase of acetonitrile/water（0.5% TFA） at a volume ratio of 25：75. At a signal to noise ratio（S/N） of 10, the detective limit of the quantitation of hippuric acid was （0.4600±0.0097） μmol/L. The standard curve shows a linear response with a slope of 49488 and a correlation coefficient of 0.9995. The assay was adequate for the study of ACE inhibition by Captopril and peptides derived from food protein, and showed a very good correlation with the previous methods.A rapid, simple and interference-free method was developed to evaluate the inhibitory activity of hydrolyzed peptides from egg white protein against the angiotensin-converting enzyme. The total reaction volume was 60 μL, saving the cost. The assay was based on a HPLC separation and quantification of the synthetic substrate hippuryl-L-histidyl-L-leucine and its hydrolyzed product-hippuric acid; the separation was performed on a C18 column eluted by a mobile phase of acetonitrile/water（0.5% TFA） at a volume ratio of 25：75. At a signal to noise ratio（S/N） of 10, the detective limit of the quantitation of hippuric acid was （0.4600±0.0097） μmol/L. The standard curve shows a linear response with a slope of 49488 and a correlation coefficient of 0.9995. The assay was adequate for the study of ACE inhibition by Captopril and peptides derived from food protein, and showed a very good correlation with the previous methods.

关 键 词：Angiotensin converting enzyme HPLC Egg white protein PEPTIDE 

分 类 号：TQ577.13[化学工程—精细化工] Q555[生物学—生物化学]