Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris  

作　　者：ZHANG Ai-li LU Jia-hui ZHANG Na ZHENG Dan ZHANG Gui-rong TENG Li-rong 

机构地区：[1]College of Life Science, Jilin University, Changchun 130012, P R. China

出　　处：《Chemical Research in Chinese Universities》2010年第5期798-802,共5页高等学校化学研究（英文版）

基　　金：Supported by the Xiuzheng Pharmaceutical Group Ltd. Commercial Project, China(No.2007220101002580)

摘　　要：A water-soluble polysaccharide（named MCMP） was isolated from the mycelium with high yield mutation Cordyceps militaris by hot-water extraction, deproteinization by sevage, alcohol precipitation, anion-exchange and gel filtration chromatography CL-6B. The polysaccharide contained mannose, rhamnose, galactose and glucose in a molar ratio of 59.36：1：8.31：39.50, of which the average molecular weight is 8100. In our research, Hep-G2 cells, Hela cells and mesangial cells were chosen to determine the anti-tumor activity of the polysaccharide. The results of MTT assay show that polysaccharides of the mutant strain presented inhibitory activity on the cells proliferation after 48 h incubation.A water-soluble polysaccharide（named MCMP） was isolated from the mycelium with high yield mutation Cordyceps militaris by hot-water extraction, deproteinization by sevage, alcohol precipitation, anion-exchange and gel filtration chromatography CL-6B. The polysaccharide contained mannose, rhamnose, galactose and glucose in a molar ratio of 59.36：1：8.31：39.50, of which the average molecular weight is 8100. In our research, Hep-G2 cells, Hela cells and mesangial cells were chosen to determine the anti-tumor activity of the polysaccharide. The results of MTT assay show that polysaccharides of the mutant strain presented inhibitory activity on the cells proliferation after 48 h incubation.

关 键 词：Cordyceps militaris POLYSACCHARIDE ANTI-TUMOR 

分 类 号：TQ463.5[化学工程—制药化工] TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]