凝血酶对A549细胞增殖和顺铂凋亡效应影响的实验研究  

Effect of thrombin on Non-small cell lung carcinoma(A549)proliferation and PDD-induced cell apoptosis in Vitro

作　　者：王盛兰[1] 王熙才[2] 伍治平[2] 金从国[2] 陈晓群[2] 蒋永新[2] 谷玉兰[2] 陈艳[2] 周永春[2] 

机构地区：[1]云南省第一人民医院呼吸二科,云南昆明650000 [2]昆明医学院第三附属医院肿瘤研究所,云南昆明650118

出　　处：《现代肿瘤医学》2010年第10期1919-1921,共3页Journal of Modern Oncology

摘　　要：目的:研究凝血酶在体外对非小细胞肺癌细胞(A549)增殖、化疗药物顺铂疗效的影响及其机理。方法:非小细胞肺癌细胞株A549与不同浓度的凝血酶(0.05-3U/ml)共培养,按不同分组加入顺铂(30mg/ml)和水蛭素(0.05-10U/ml)。在不同的时间,采用MTT比色法检测细胞生长活性,细胞增殖程度;采用流式细胞术检测凝血酶对顺铂杀伤肿瘤细胞作用的影响。结果:凝血酶在一定浓度范围内(0.05-1.5 U/ml)可促进A549增殖,72 h后增殖效应明显;在增殖浓度范围内可抑制顺铂对肿瘤细胞A549的凋亡作用。结论:凝血酶在一定浓度范围内诱导A549细胞增殖,并减弱化疗药物顺铂对该细胞的杀伤作用。Objective：To study the effect of thrombin for cell proliferation and inhibition of cisplatin（PDD）-induced cell apoptosis on the human non-small cell lung cancer cells line A549 in vitro,and explore its mechanism.Methods： Cell proliferation in different concentration and time was determined by improved MTT colorimetric assay.The fluorescence flow cytometry（FCM） DNA assay was applied to detect the changes of apoptotic rate.Results： Thrombin may increase the cell proliferation in 0.5-1.5 u/ml concentrations and decrease PDD-induced cell apoptotic rate.Conclusion： Thrombin may substantially stimulate the cell proliferation and change PDD-induced cell apoptotic rate for A549 cell in vitro.

关 键 词：凝血酶 A549 增殖 

分 类 号：R730.3[医药卫生—肿瘤]