转化生长因子-β_1联合甲胎蛋白异质体二联检测对原发性肝癌诊断的意义  

The diagnostic value of transforming growth factor-β_1 and alpha-fetoproteinL3 combined examination in hepatocellular carcinoma

作　　者：邵渊[1] 王作仁[1] 李峰[1] 

机构地区：[1]西安交通大学医学院第一医院,陕西西安710061

出　　处：《现代肿瘤医学》2010年第10期2009-2012,共4页Journal of Modern Oncology

摘　　要：目的:分析转化生长因子-β1(TGFβ1)及甲胎蛋白异质体(AFPL3)对原发性肝癌的诊断价值。方法:以酶联免疫吸附试验(ELISA)测定肝癌高危人群和原发性肝癌患者和正常对照者的血浆TGFβ1水平,以凝集素亲和免疫电泳法测定AFPL3水平,并分析TGFβ1,AFPL3联合检测在原发性肝癌诊断方面的临床意义。结果:原发性肝癌组TGFβ1水平(2.31±1.28μg/L)显著高于正常对照组(0.59±0.12μg/L),肝炎后肝硬化组(1.09±0.15μg/L),有肝癌家族史的慢性乙型肝炎组(1.06±0.17μg/L),慢性乙型肝炎组((1.05±0.16μg/L),以TGFβ1以1.2μg/L为界限,其诊断原发性肝癌的灵敏性和特异性分别为92.5%和95.0%。原发性肝癌组AFPL3水平(40.5±29.6)%,显著高于正常对照组(3.9±3.1)%,肝炎后肝硬化组(10.1±5.8)%,有肝癌家族史的慢性乙型肝炎组(9.5±6.6)%,慢性乙型肝炎组(5.5±4.5)%,正常对照组(3.9±3.1)%,以AFPL3>15%为界限,其诊断原发性肝癌的灵敏性和特异性分别为87.5%和90.3%。TGFβ1,AFPL3联合阳性率,原发性肝癌组65.0%,显著高于正常对照组0.0%,肝炎后肝硬化组3.2%,有肝癌家族史的慢性乙型肝炎组0.0%,慢性乙型肝炎组0.0%。结论:转化生长因子β1联合甲胎蛋白异质体检测对原发性肝癌的诊断有重要的意义。Objective：To study the diagnostic value of transforming growth factor-β1 and alpha-fetoproteinL3 combined examination in hepatocellular carcinoma.Methods：Plasma level of TGFβ1 expression were analyzed with enzyme-linked immunosorbent assay（ELISA） in patients with HCC,HCC high risk patients and healthy control,by using lectin affinity and immune electrophoresis to analyse alpha-fetoproteinL3（AFPL3） level.Results：The plasma TGFβ1 level in HCC patients was 2.31±1.28μg/L（mean±SD） and significantly higher（P〈0.05）than those in normal subjects（0.59±0.12μg/L）,post-hepatic inflammation accompanied hepatic fibrosis（1.09±0.15μg/L）,chronic hepatitis B with HCC family history（1.06±0.17μg/L）,chronic hepatitis B group（1.05±0.16μg/L）,the sensitivity and specificity of plasma TGFβ1 lever were 92.5%,95.0% in diagnosis of HCC with 1.2μg/L as normal up-limitation.The plasma AFPL3 level in HCC patients was（40.5±29.6）% and significantly higher（P〈0.05）than those in normal subjects（3.9±3.1）%,post hepatic inflammation accompanied hepatic fibrosis（10.1±5.8）%,chronic hepatitis B with HCC family history（9.5±6.6）%,chronic hepatitis B group（5.5±4.5）%.The sensitivity and specificity of plasma AFPL3 level were 87.5%,90.3% in diagnosis of HCC with AFPL315% as normal up-limitation.The combined positive rate in HCC patients was 65.0%,significantly higher than those in normal subjects 0.0%,post hepatic inflammation accompanied hepatic fibrosis 3.2%,chronic B hepatitis with HCC family history 0.0%,chronic B hepatitis group 0.0%.Conclusion：Transforming growth factor-β1 and alpha-fetoproteinL3 combined examination is of significantly diagnostic value in HCC.

关 键 词：转化生长因子Β1 甲胎蛋白异质体 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]