HLA-DR抗原及HPV16/18E6蛋白在宫颈癌的表达及其相互关系  

Expression and correlation of HLA-DR antigen and HPV16/18E6 protein in cervical cancer

作　　者：黄金双[1,2] 齐跃[1] 林蓓[1] 王冬冬[1] 周丹[1,3] 董伟妍[1,4] 张帆[1,5] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]宁波市妇女儿童医院妇产科,浙江宁波315012 [3]卫生部北京医院妇产科,北京100730 [4]中国医科大学北京顺义医院妇产科,北京101300 [5]北京市海淀区妇幼保健院妇产科,北京100080

出　　处：《现代肿瘤医学》2010年第10期2023-2026,共4页Journal of Modern Oncology

基　　金：辽宁省攻关项目(编号:2003225004);辽宁省攻关项目(编号:2005225007-2);沈阳市科技攻关项目(编号:1053123-1-54-1);辽宁省科技攻关项目(编号:20121268)

摘　　要：目的:通过检测HLA-DR抗原及HPV16/18E6蛋白在宫颈癌组织中的表达情况,探讨由HLA-DR抗原介导的免疫应答在感染高危型HPV至发展为宫颈癌过程中的作用机制。方法:采用免疫组织化学SP法检测宫颈癌、CIN及正常宫颈组织中HLA-DR抗原及HPV16/18E6的表达情况。结果:HLA-DR抗原的阳性表达率在宫颈癌、CIN、正常宫颈组织中分别为81.8%、73.3%、37.5%,而HPV16/18E6蛋白的阳性表达率则分别为75.8%、60.0%、37.5%,两者在三组中的表达均有显著差异(P均(0.05)。HLA-DR抗原及HPV16/18E6蛋白在宫颈癌组织中的阳性表达率随临床分期、分化程度变化及淋巴结是否转移而不同,但其差异无显著意义(P均(0.05)。结论:HLA-DR抗原递呈病毒抗原给T细胞引起的免疫应答可能在宫颈癌的发生发展过程中起着重要的作用。Objective：To investigate the role of immune response mediated by HLA-DR antigen in the process of from high-risk HPV infection to cervical cancer by detecting the expression of HLA-DR antigen and HPV16/18E6 protein in cervical cancer.Methods：Immunohistochemistry SP was used to examine the expression of HLA-DR antigen and HPV16/18E6 protein in samples of normal cervix,CIN and cervical cancer.Results：The rates of positive expression for HLA-DR antigen in tissue of cervical cancer,CIN and normal cervix were 81.8%,73.3% and37.5%,respectively.While the rates of positive expression for HPV16/18E6 protein were 75.8%,60.0% and 37.5%.Both showed significant differences in the three groups（P﹤0.05）.The expression of HLA-DR antigen and HPV16/18E6 protein in cervical cancer tissue was different in clinical stages,degrees of differentiation and lymphoid node metabasis,but no significant differences exist between them（P﹥0.05）.Conclusion：The immune response resulted from HLA-DR antigen presenting viral antigen to T cells may play an important role in carcinogenesis of cervical cancer.

关 键 词：HLA-DR抗原 HPV16/18E6蛋白 宫颈癌 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]