加载牵张应力对人牙周膜成纤维细胞生长和增殖的影响  

Effects of different tension force on the growth and proliferation of HPDLF

作　　者：张晓东[1] 李永明[2] 宋九余[1] 林珠[2] 

机构地区：[1]沈阳军区总医院口腔内科.辽宁省口腔医学研究所,辽宁沈阳110840 [2]第四军医大学口腔医学院正畸科,陕西西安710032

出　　处：《临床口腔医学杂志》2010年第8期464-467,共4页Journal of Clinical Stomatology

基　　金：辽宁省博士启动基金资助(20071040)

摘　　要：目的:探讨不同力值和不同频率的牵张应力对HPDLF增殖的影响。方法:通过细胞牵张应力加载系统,施加频率为0.1Hz,大小为6%、12%、18%形变率的牵张应力;大小为12%形变率的频率为0.1Hz、0.2Hz及持续性的牵张应力,利用四唑盐(MTT)比色试验观察牵张应力对HPDLF增殖的影响。结果:频率为0.1Hz时,6%、12%的牵张应力对HPDLF有促增殖作用,12%的牵张力作用最强,18%的牵张应力对HPDLF的增殖有抑制作用;大小为12%时,0.2Hz的牵张应力对HPDLF的促增殖作用最强,持续性牵张应力促增殖作用最小。结论:不同力值和频率的牵张应力对HPDLF的促增殖作用影响不同,12%形变率、频率为0.2Hz的牵张应力有最佳的促增殖效果。Objective：To investigate the effects of the different mechanical strain on the proliferation of HPDLF. Method：HPDLF were subjected to 6 %,12 %,18 % elongation by stretching frequency 0.1 Hz,or 12 % elongation by stretching frequency 0.1 Hz,0.2 Hz and sustained stretching force in this experiment. MTT was performed to observe the proliferation of HPDLF. Result：The frequency 0.1 Hz stretching force could promote HPDLF proliferation at 6 %,12 % elongation and the 12 % elongation is more powerful. Stretching force could restrain HPDLF proliferation at 18 % elongation by frequency 0.1Hz. At 12 % elongation,the best stretching frequency for HPDLF proliferation was 0.2 Hz and the least was sustained stretching force. Conclusion：Different stretching force had different effects on the proliferation of HPDLF and the stretching force of 12 % elongation by frequency 0.2 Hz was the best.

关 键 词：牵张应力 牙周膜成纤维细胞 生长 增殖 

分 类 号：R783.5[医药卫生—口腔医学]