多巴胺-β-羟化酶基因多态性与电焊工神经功能异常易感性的关系  

Association between polymorphism of dopamine β-hydroxylase and neurological dysfunction hereditary susceptibility of electric welders

作　　者：陈雯雯[1] 邵华[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院职业卫生检测评价中心,济南250062

出　　处：《中华劳动卫生职业病杂志》2010年第9期656-659,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家“十一五”科技支撑项目（2006BAT061302）

摘　　要：目的 研究多巴胺-β-羟化酶（DBH）基因多态性与锰所致神经损伤遗传易感性的关系.方法 采用横断面研究设计,选择锰浓度稳定、工人作业位置相对固定、工作1年以上的专职电焊作业人员402名锰接触工人为对象,根据累积接触指数（CEI）分为低接触组（CEI〈1）和高接触组（CEI＞1）,在高、低接触组间,根据神经系统检查结果将接触人群分为神经功能正常组和异常组,高接触组中,神经功能异常者81名,低接触组中神经功能异常者28名,差异有统计学意义（P〈0.05）.应用聚合酶链反应-限制性长度片段多态性（PCR-RFLP）分析技术进行DBH基因型分析.结果 在神经功能异常组与正常组间,DBH基因A2A2型与A2等位基因分布明显不同.在高接触组中,神经功能异常者DBH的A2A2基因型和A2等位基因频率明显高于正常组,差异有统计学意义（A2A2型OR=1.248,P〈0.05;A2等位基因OR=1.103,P〈0.05）;在低接触组中,神经功能异常者DBH的A2等位基因频率明显高于正常组,差异有统计学意义（OR=1.176,P〈0.05）.结论 携带DBH基因A2A2型与A2等位基因的个体接触锰达到一定时间发生神经功能异常的危险性会增高,DBH基因（intron 5 Taq Ⅰ）多态性可能在锰致神经功能异常的遗传易感性中起作用.Objective To investigate the relationship between genetic polymorphism of dopamine β-hydroxylase（DBH） and manganese-induced nerve injury. Methods In a cross-sectional study, 402 electric welders who had worked over one year in relatively fixed sites were recruited, and the concentration of manganese in which they worked was stable. These samples was divided into high exposure group（CEI〉1 ） and low exposure group （CEI〈1） by CEI. Between the two groups, the groups were divided into abnormal group and normal group according to the result of neurologic check （there were 81 workers with abnormal neunological dysfunction in high exposure group and 28 workers in low exposure group, P〈0.05）. Polymorphism of DBHgene was analyzed with polymerase chain reaction-restriction fragment length polymorphism. Results The distribution of A2A2 genotype and A2 allele of DBH was significantly different. In high exposure group, the distribution of A2A2 genotype and A2 allele of DBH in abnormal group was significantly wider than in normal group（A2A2 genotype, OR=1.248, P〈0.05, A2 allele, OR=1. 103 ,P〈0.05 ）. In low exposure group, the distribution of A2 allele of DBH in abnormal group was significantly wider than in normal group （OR=1.176,P〈0.05 ）. Conclusion The individuals who carry A2A2 genotype and A2 allele of DBH have increased risk of neunological dysfunction after explosion to manganese for a certain time, which suggests that polymorphism of DBH （intron 5 Taq Ⅰ ） would play a great role in hereditary susceptibility of neunological dysfunction cause by manganese.

关 键 词：锰 多巴胺Β羟化酶 多态性 单核苷酸 疾病遗传易感性 

分 类 号：R686[医药卫生—骨科学]