凋亡抑制基因Livin在人胃癌组织中的表达及临床意义  

Expression of Livin in gastric dancer and its clinical significance

作　　者：张健[1] 王雪松[1] 李洪运[1] 董向[1] 解瑞金[1] 

机构地区：[1]济宁市第一人民医院,山东济宁272100

出　　处：《实用肿瘤杂志》2010年第5期546-549,共4页Journal of Practical Oncology

摘　　要：目的研究凋亡抑制基因Livin在人胃癌组织中的表达及其与组织分化程度、淋巴结转移等临床病理参数之间的关系。方法应用RT-PCR方法检测Livinα和Livinβ mRNA表达水平,卡方检验分析两组之间表达水平差异。将胃癌临床病理参数与表达水平进行等级相关分析和检验,探讨二者之间的相关性。结果 40例胃癌患者中,Livin表达阳性率52.5%,且Livinα和Livinβ两种异构体均见表达。20例正常胃组织中均未见Livin mRNA表达,阳性率0%(P<0.05)。Livin的表达与胃癌的分化程度以及是否有淋巴结转移之间比较差异有统计学意义(P<0.05)。相关性分析示Livin的表达与胃癌的浸润深度、TNM分期之间呈正相关,但Livin的表达差异无统计学意义(P>0.05)。Livin的表达与年龄、性别、胃癌的生长部位差异均无统计学意义(P>0.05)。结论 Livin在胃癌组织中特异性的表达,可能作为胃癌免疫及基因治疗的一个有效靶点。Objective To detect the expression of Livin in human gastric carcinoma and to analyze the relationship between Livin expression and cliniopathologic features.Methods The expression levels of Livinα and Livinβ mRNA were determined by RT-PCR.The relationship between Livin expression and clinicopathologic features was analyzed.Results The expressions of both Livinα and Livinβ mRNA were detected in 21 of 40 gastric carcinoma cases（52.5%）.No expression of Livin was detected in 20 cases of benign gastric lesion.A positive correlation was found between Livin expression and poor differentiation as well as lymph node metastases（P0.05）.No significant influence was detected by age,sex,and growth position of the expression of Livin in gastric carcinoma.Conclusions Livin is expressed in gastric carcinoma especially with a correlation to tumor differentiation and lymph node metastasis.Livin might serve as a new target for apoptosis-inducing therapy of gastric cancer.

关 键 词：胃肿瘤 细胞凋亡 基因 Livin 逆转录聚合酶链反应 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]