沈阳地区肺炎支原体23SrRNA基因突变初步研究  

Mutations of the 23SrRNA gene of Mycoplasma pneumoniae in Shenyang

作　　者：路素坤[1] 韩晓华[1] 李书琴[1] 陈宁[1] 李书秀[1] 

机构地区：[1]中国医科大学附属盛京医院小儿呼吸科,沈阳110817

出　　处：《中国卫生检验杂志》2010年第10期2550-2552,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解沈阳地区的肺炎支原体(Mycoplasma pnemoniae,MP)23SrRNA基因的突变现状,为进一步认识突变MP所致肺炎支原体肺炎(MPP)的临床表现及其治疗打下基础。方法:对180例疑似MPP住院患儿咽拭子标本提取MP-DNA应用荧光探针定量PCR(FQ-PCR)技术进行分子鉴定,并于患儿病程7天后采血,应用颗粒凝胶法(PA)进行肺炎支原体抗体测定;对两者均阳性标本应用巢式PCR扩增23SrRNA基因并进行电泳检测及DNA测序分析,将测得序列与基因库中MP标准株基因序列进行比较。结果:180例临床标本荧光探针定量和肺炎支原体抗体均确定阳性52例,应用巢式PCR技术扩增MP-23SrRNA基因,并对扩增产物进行测序,测出45例,45例23SrRNA中均存在2063位A→G的点突变,其中2例为2063位点A、G碱基共存,考虑为敏感株与耐药株共生,突变率100%。结论:沈阳地区MP23SrRNA基因中普遍存在点突变,突变MP与患儿的临床特点有何联系有待于进一步研究。Objective：To study mutations of the 23SrRNA gene of Mycoplasma pneumoniae（MP） in ShenYang and provide foundation for further study of clinical situation and clinical therapy of patients infected with it.Methods： Fluorescence quantitative polymerase chain reaction（FQ-PCR）were adopted to detect DNA of MP in 180 patients with suspected Mycoplasma pneumoniae pneumonia（MPP）,MP-23SrRNA gene was detected by nested PCR and DNA sequencing.the DNA sequences were compared with the corresponding sequence of MP reference strain in genebank.Results： Of the 180 clinical specimens,52 were identified by FQ-PCR and 45 were sequenced.All had A to G mutation at position 2063,in which 2 showed the coexistence of A and G,the sensitive strain and the resistant strain.the percentage of mutation was 100%.Conclusion： The point mutation of the 23SrRNA region Ⅴ is commomn in shen yang,the relationship between mutational MP and clinical manifestation of patients need further research.

关 键 词：肺炎支原体 23SrRNA 基因突变 聚合酶链反应 

分 类 号：R563.1[医药卫生—呼吸系统]