市售海虾副溶血性弧菌多血清群混合污染状况研究  

Analysis on mixed pollution status of Vibrio parahaemolyticus in sea shrimp on the market

作　　者：叶菊莲[1] 梅玲玲[1] 罗芸[1] 占利[1] 徐宝祥[1] 朱敏[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2010年第10期2591-2592,2594,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解市售海虾类副溶血性弧菌(Vibrio parahaemolyticus,VP)污染状况,为浙江省预防控制副溶血性弧菌食源性疾病提供技术支撑。方法:选择夏冬两个季节,从杭州市15个农贸市场采集基围虾、明虾、白虾。采用MPN法定量检测副溶血性弧菌,参照GB/T4789.7-2008方法分离、鉴定副溶血性弧菌,采用血清凝集试验对菌株进行血清型分析。结果:78件海虾74件检出副溶血性弧菌,副溶血性弧菌检出率高达94.9%。不同的季节其检出率无显著差异(P>0.05),但夏季副溶血性弧菌的污染浓度显著高于冬季(P<0.001);血清群集中在O1、O5、O11,三个血清型共占总分离菌株数的51.3%。多种O抗原群副溶血性弧菌混合污染情况夏季明显高于冬季(P<0.005)。结论:本省市售虾类副溶血性弧菌污染情况十分严重,夏季尤其突出,因此加强市售海虾类副溶血性弧菌的监测,对控制副溶血性弧菌食物中毒或食源性疾病发生有着重要的意义。Objective：To define the Vibrio parahaemolyticus（V.parahaemolyticus） polluted status among sea shrimps from agricultural markets in hangzhou.Methods： Samples such as greasyback shrimp,prawn and white shrimp from 15 agricultural markets in hangzhou were collected during summer and autumn.Quantitative analysis,isolation and culture,biochemical identification and serotyping were carried out according to GB/T4789.7-2008.Results： Out of 78 sea shrimps,74 were found containing Vibrio parahaemolyticus with the positive rate of 94.9%.Incidence of V.parahaemolyticus among different seasons were close（P0.05）,while the contamination density of V.parahaemolyticus in Summer was significantly higher than that of in winter（P0.001）.Of these Vibrio parahaemolyticus isolates,the dominating serogroups are O1,O5 and O11 with a total share rate of 51.3%.Mixed contamination rate of various group O antigen of V.parahaemolyticus in summer was significantly higher than that of in winter（P0.005）.Conclusion： The pollution of V.parahaemolyticus in sea shrimp is very serious in Zhejiang province,particularly in summer.Monitoring of V.parahaemolyticus in sea shrimps should be strengthened.It is of great significance to control Vibrio parahaemolyticus food poisoning or food borne diseases.

关 键 词：副溶血性弧菌 定量监测 血清群 

分 类 号：R378.3[医药卫生—病原生物学]